State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences and Institute of Biomedical Sciences, Fudan University, Shanghai 200032, China.
Science. 2010 Feb 19;327(5968):1004-7. doi: 10.1126/science.1179687.
Lysine acetylation regulates many eukaryotic cellular processes, but its function in prokaryotes is largely unknown. We demonstrated that central metabolism enzymes in Salmonella were acetylated extensively and differentially in response to different carbon sources, concomitantly with changes in cell growth and metabolic flux. The relative activities of key enzymes controlling the direction of glycolysis versus gluconeogenesis and the branching between citrate cycle and glyoxylate bypass were all regulated by acetylation. This modulation is mainly controlled by a pair of lysine acetyltransferase and deacetylase, whose expressions are coordinated with growth status. Reversible acetylation of metabolic enzymes ensure that cells respond environmental changes via promptly sensing cellular energy status and flexibly altering reaction rates or directions. It represents a metabolic regulatory mechanism conserved from bacteria to mammals.
赖氨酸乙酰化调节许多真核细胞的过程，但它在原核生物中的功能在很大程度上是未知的。我们证明了沙门氏菌中的中心代谢酶在响应不同碳源时会广泛且不同地乙酰化，同时伴随着细胞生长和代谢通量的变化。控制糖酵解与糖异生方向以及柠檬酸循环与乙醛酸支路分支的关键酶的相对活性都受到乙酰化的调节。这种调节主要由一对赖氨酸乙酰转移酶和去乙酰化酶控制，它们的表达与生长状态相协调。代谢酶的可逆乙酰化确保细胞通过迅速感知细胞能量状态并灵活改变反应速率或方向来响应环境变化。这代表了从细菌到哺乳动物的一种保守的代谢调节机制。
