Department of Medicinal Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
J Pharmacol Sci. 2010;112(3):282-9. doi: 10.1254/jphs.09225fp. Epub 2010 Feb 18.
The present study was undertaken to investigate changes of the electroencephalogram (EEG) induced by pentetrazol (PTZ) in comparison with behavioral seizures in mice. Under pentobarbital anesthesia, mice were fixed to a stereotaxic apparatus, and electrodes were implanted into the frontal and occipital cortex. Behavioral and EEG changes were observed for 30 min following PTZ administration. After PTZ administration, mice showed myoclonic seizure (MCL) and clonic seizure (CL) in order. At the same time, spiking activity and spike-wave discharge in the cortex were observed. Phenobarbital, sodium valproate, diazepam, ethosuximide, and gabapentin caused a dose-dependent shortening of the duration of MCL and CL. In addition, they shortened the duration of spiking activity and spike-wave discharge dose-dependently. Moreover, phenytoin significantly inhibited the duration of spiking activity. It can be concluded that PTZ-induced spiking activity and spike-wave discharge serve as useful indices to assess the potential of antiepileptic activity in absence and MCLs in humans. Moreover, it is supposed that employing an index of EEG activity in addition to that of behavioral activity is desirable for objectivity.
本研究旨在探讨戊四氮（PTZ）诱导的脑电图（EEG）变化与小鼠行为性癫痫发作的关系。在戊巴比妥麻醉下，将小鼠固定在立体定向仪上，并将电极植入额皮质和枕皮质。在给予 PTZ 后观察 30 分钟的行为和 EEG 变化。给予 PTZ 后，小鼠依次出现肌阵挛性发作（MCL）和阵挛性发作（CL）。同时，观察到皮质中的尖峰活动和尖波放电。苯巴比妥、丙戊酸钠、地西泮、乙琥胺和加巴喷丁剂量依赖性地缩短 MCL 和 CL 的持续时间。此外，它们还剂量依赖性地缩短尖峰活动和尖波放电的持续时间。此外，苯妥英钠显著抑制尖峰活动的持续时间。可以得出结论，PTZ 诱导的尖峰活动和尖波放电可作为评估人类失神和 MCL 抗癫痫活性潜力的有用指标。此外，除了行为活动的指标外，采用 EEG 活动的指标更具客观性。
