Tachibana M, Deguchi N, Jitsukawa S, Baba S, Hata M, Tazaki H
Department of Urology, School of Medicine, Keio University, Tokyo, Japan.
J Urol. 1991 May;145(5):963-7. doi: 10.1016/s0022-5347(17)38501-4.
A total of 56 human bladder tumors that were histologically proved to be transitional cell carcinoma was analyzed by simultaneous flow cytometric 2-color measurements of deoxyribonucleic acid (DNA) and bromodeoxyuridine. Bromodeoxyuridine in vitro labeling was performed by sample incubation with bromodeoxyuridine under high atmospheric pressure oxygen. Grade 1 tumors showed 33.3% aneuploidy with a mean bromodeoxyuridine positive stained ratio (labeling index) of 5.1 +/- 3.4%. Grade 2 tumors featured 51.7% aneuploidy with a mean labeling index of 8.9 +/- 7.7%. On the other hand, a markedly increased labeling index of 15.2 +/- 8.2% with aneuploidy was observed in all but 1 grade 3 tumors. When DNA ploidy and labeling indexes were compared according to the presence or absence of muscular invasion of tumors, all 16 muscle invasive tumors showed aneuploidy (mean labeling index 18.7 +/- 8.0%), while 17 of 40 nonmuscle invasive tumors showed aneuploidy (mean labeling index 8.6 +/- 5.4%). This labeling index difference was statistically significant (p less than 0.01). These results indicate that bromodeoxyuridine/DNA 2-color flow cytometry may provide an objective parameter for quantification of the malignant potential of bladder cancers.
对56例经组织学证实为移行细胞癌的人类膀胱肿瘤进行了脱氧核糖核酸（DNA）和溴脱氧尿苷的同步流式细胞仪双色测量分析。通过在高气压氧气条件下将样本与溴脱氧尿苷一起孵育来进行溴脱氧尿苷体外标记。1级肿瘤显示33.3%的非整倍体，平均溴脱氧尿苷阳性染色率（标记指数）为5.1±3.4%。2级肿瘤的特征是非整倍体占51.7%，平均标记指数为8.9±7.7%。另一方面，除1例3级肿瘤外，所有3级肿瘤均观察到非整倍体且标记指数显著升高，为15.2±8.2%。当根据肿瘤是否存在肌肉浸润比较DNA倍体和标记指数时，所有16例肌肉浸润性肿瘤均显示非整倍体（平均标记指数18.7±8.0%），而40例非肌肉浸润性肿瘤中有17例显示非整倍体（平均标记指数8.6±5.4%）。这种标记指数差异具有统计学意义（p<0.01）。这些结果表明，溴脱氧尿苷/DNA双色流式细胞术可能为量化膀胱癌的恶性潜能提供一个客观参数。
