Department of Odontostomatological and Maxillo Facial Sciences, Federico II University of Naples, Italy.
Acta Derm Venereol. 2010 Mar;90(2):115-21. doi: 10.2340/00015555-0819.
Bullous pemphigoid is associated with antibodies to a 230 kDa and a 180 kDa protein. In a literature review we investigated the role of auto-antibodies as detected by different serological assays. Nine reports containing data on 143 patients were analyzed. Pre-treatment data showed that indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA) and immunoblotting offer an 82.2% or greater probability of being positive. At the end of the study period, all patients had clinically improved, whether or not they were on therapy. Auto-antibodies were present in 29% of patients evaluated by monkey esophagus immunofluorescence and 75% of those evaluated by human skin immunofluorescence. Positive titers were also reported in 67.6% of patients evaluated by ELISA. In 100% of patients in whom immunoblotting was performed the titers became negative. In 3 patients (5.3%) using human skin immunofluorescence and in one patient (1.4%) using ELISA the titers were increased at the end of the study period. The correlation between anti-basement membrane zone antibodies and the clinical course of bullous pemphigoid requires further and long-term studies.
大疱性类天疱疮与针对 230 kDa 和 180 kDa 蛋白的抗体有关。在文献回顾中，我们研究了不同血清学检测方法所检测到的自身抗体的作用。分析了 9 份包含 143 例患者数据的报告。预处理数据表明，间接免疫荧光、酶联免疫吸附试验（ELISA）和免疫印迹检测的阳性率为 82.2%或更高。在研究结束时，所有接受治疗的患者的临床症状均有改善。通过猴食管免疫荧光检测，29%的患者存在自身抗体，通过人皮肤免疫荧光检测，75%的患者存在自身抗体。ELISA 检测的阳性滴度也有 67.6%的报告。在进行免疫印迹检测的 100%的患者中，滴度变为阴性。在 3 名（5.3%）使用人皮肤免疫荧光和 1 名（1.4%）使用 ELISA 的患者中，研究结束时滴度增加。基底膜带抗体与大疱性类天疱疮的临床病程之间的相关性需要进一步和长期的研究。
