研究 T-2 毒素联合低营养饮食对大鼠骺板生长发育的影响。
Study on the effect of T-2 toxin combined with low nutrition diet on rat epiphyseal plate growth and development.
机构信息
Orthopedic Department, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
出版信息
Int Orthop. 2010 Dec;34(8):1351-6. doi: 10.1007/s00264-010-0966-z. Epub 2010 Feb 19.
Abstract
The purpose of this study was to observe early lesions of rat epiphyseal plates and metaphysis caused by T-2 toxin and T-2 toxin combined with a low nutrition diet to determine possible pathogenic factors of Kashin-Beck disease (KBD). Ninety Wistar rats were divided into three groups. Group A was fed with a normal diet as control; group B was fed with a normal diet and T-2 toxin; and group C was fed with a low nutrition diet and T-2 toxin. The left knee specimens were collected, fixed in formaldehyde solution, stained by hematoxylin and eosin and Masson. After two weeks, the epiphyseal plate showed necrosis of chondrocytes in groups B and C. After four weeks, more obvious chondrocyte necrosis appeared. The positive rate of Lamellar necrosis in group C was significantly higher than that in groups B and A (P < 0.01). Metaphyseal trabecular bone showed sparse disorder and disruption in group C. T-2 toxin combined with a low nutrition diet could lead to more serious chondrocyte necrosis in the epiphyseal plate and disturb metaphyseal trabecular bone formation.
摘要
本研究旨在观察 T-2 毒素及 T-2 毒素联合低营养饮食对大鼠骺板和骺软骨早期病变的影响，以探讨大骨节病（KBD）的可能致病因素。90 只 Wistar 大鼠分为三组，A 组为正常饮食对照组，B 组为正常饮食加 T-2 毒素组，C 组为低营养饮食加 T-2 毒素组。取左侧膝关节标本，福尔马林溶液固定，HE 和 Masson 染色。2 周后，B、C 组骺板出现软骨细胞坏死，4 周后软骨细胞坏死更加明显，C 组层状坏死阳性率明显高于 B 组和 A 组（P<0.01），C 组骺板骨小梁稀疏紊乱。T-2 毒素联合低营养饮食可导致骺板软骨细胞坏死更加严重，并干扰骺板骨小梁形成。
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