University of Minnesota, St. Paul, MN 55108, USA.
Appl Biochem Biotechnol. 2010 Nov;162(5):1286-96. doi: 10.1007/s12010-010-8914-3. Epub 2010 Feb 19.
The effect of pH on hydrogen production from liquid swine manure supplemented with glucose by a mixed culture of fermentative bacteria in an anaerobic sequencing batch reactor was evaluated in this study. At 37 +/- 1 degrees C, five pH values ranging from 4.7 to 5.9 at an increment of 0.3 were tested at a hydraulic retention time (HRT) of 16 h. The results showed that at this HRT, the optimal pH for hydrogen production was 5.0, under which the biogas comprised 33.57 +/- 5.65% of hydrogen with a production rate of 8.88 +/- 2.94 L-H(2)/day and a yield of 1.48 +/- 0.49 L-H(2)/L liquid swine manure. The highest biomass concentration, highest butyric acid to acetic acid ratio, lowest propionic acid concentration, and the best stability were all found at pH 5.0, while the highest CH(4) productivity was found at pH 5.9. For efficient hydrogen production, oxygen content should be controlled under 2%, beyond which an inverse linear relationship (R(2) = 0.986) was observed.
本研究考察了在厌氧序批式反应器中，混合发酵细菌以葡萄糖为补充物，从液态猪粪制取氢气过程中 pH 值的影响。在 37°C（37 摄氏度）、水力停留时间（HRT）为 16 小时的条件下，设置 pH 值范围为 4.7 至 5.9，每 0.3 个单位递增，共 5 个梯度。结果表明，在此 HRT 条件下，最佳产氢 pH 值为 5.0，在此 pH 值下，沼气中氢气含量为 33.57%±5.65%，氢气日产量为 8.88L±2.94L，氢气产率为 1.48L±0.49L 液态猪粪。在 pH 值为 5.0 时，生物量浓度最高、丁酸与乙酸比值最高、丙酸浓度最低，且稳定性最好，而在 pH 值为 5.9 时，CH4 产率最高。为实现高效产氢，需将氧含量控制在 2%以下，在此条件下，观察到氧含量与氢气产量呈显著的负线性关系（R2=0.986）。
