European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, B-2440 Geel, Belgium.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 Jun;27(6):793-800. doi: 10.1080/19440040903551939.
The performance characteristics of a method for the determination of the marker substance glycerol triheptanoate (GTH) in processed animal by-products (ABPs) based on gas chromatography (GC) coupled to mass spectrometry (MS) were determined via a collaborative study. Within the European Union, GTH needs to be added to the portion of processed ABPs that must not enter the feed and food chain (Categories 1 and 2) at a minimum concentration of 250 mg kg(-1) related to the fat fraction of the test samples analysed. The test materials included in the validation study consisted of three meat and bone meal (MBM) and three fat samples that contained GTH at different concentrations ranging from 61 to 455 mg kg(-1). The relative standard deviation of repeatability (RSD(r)) varied from 3.4 to 7.8% and the relative standard deviation of reproducibility (RSD(R)) varied from 9.0 to 16.5%, corresponding to HORRAT values that were, in all cases, equal or below the critical value of 2.0. The estimated trueness expressed in terms of average concentration compared to the target concentrations of GTH in all test materials varied from 95 to 107%, confirming acceptable values for the trueness of the method. Based on the acceptable values for precision and trueness, the method is fit for the intended purpose and can be used for official control purposes to determine GTH in processed animal by-products from Category 1 and Category 2.
采用协作研究的方法，对基于气相色谱（GC）-质谱联用（MS）测定加工动物副产品（ABPs）中甘油三庚酸酯（GTH）标志物的方法的性能特征进行了确定。在欧盟，GTH 必须添加到不得进入饲料和食物链的加工 ABPs 部分（类别 1 和 2）中，其最低浓度与分析测试样品脂肪部分相关，为 250mg/kg。验证研究中包含的测试材料包括三种肉骨粉（MBM）和三种脂肪样品，其中含有不同浓度的 GTH，范围为 61 至 455mg/kg。重复性（RSD(r)）的相对标准偏差为 3.4%至 7.8%，再现性（RSD(R)）的相对标准偏差为 9.0%至 16.5%，相应的 HORRAT 值在所有情况下均等于或低于 2.0 的临界值。以所有测试材料中 GTH 的目标浓度表示的平均浓度计算的估计准确性从 95%到 107%不等，证实了该方法准确性的可接受值。基于可接受的精密度和准确性值，该方法适用于预期目的，可以用于官方控制目的，以确定来自类别 1 和类别 2 的加工动物副产品中的 GTH。
