Debski B, Gralak M, Gronowska-Senger A, Górnicka M
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.
Pol J Vet Sci. 2009;12(4):449-54.
Male rats of Wistar strain (n = 48) were fed a vitamin A deficient diet for 3 days of adaptation period and then a 10 day experimental period to reduce slightly the body stores of this vitamin. Half of the animals were subjected to physical training and/or oral vitamin A supplementation. Four different doses of supplementation were used--0, 7.5, 15 and 60 microg/d/rat, which is equivalent to 0, 25, 50 and 200 IU of vitamin A, respectively. Animals from the defined groups ran on a treadmill with a rate of 2.0 m/s for 15 minutes per day for 10 days. After overnight fasting, the rats were sacrificed, and insulin in blood serum and hepatic retinol concentrations were estimated. Daily feed intake and daily body gains were similar in groups of sedentary and physically trained rats. A moderate level of oral vitamin A supplementation (the highest supplemented dose was about 6 x the above recommended NRC level) did not cause any changes in these zootechnical parameters. Oral Vitamin A supplementation resulted in an increase in retinol concentration in the liver (F = 15.2, p < 0.001), but without significant difference between trained and untrained animals. Physical training of rats caused a statistically significant decrease of insulin concentration in blood serum (1.53 +/- 0.18 vs. 1.73 +/- 0.20). This difference was highly significant (F = 11.1, p < 0.001). Vitamin A supplementation was found not to influence the concentration of this hormone, which is responsible for energy metabolism regulation in the body. Based on estimated parameters, the necessity of vitamin A excessive use in physically trained subjects was not proven.
选用Wistar品系雄性大鼠（n = 48），在适应期给予维生素A缺乏饮食3天，然后进行为期10天的实验期，以略微降低该维生素的体内储备。将一半动物进行体育训练和/或口服补充维生素A。使用了四种不同剂量的补充剂——0、7.5、15和60微克/天/只大鼠，分别相当于0、25、50和200国际单位的维生素A。确定组别的动物在跑步机上以2.0米/秒的速度每天跑15分钟，持续10天。过夜禁食后，处死大鼠，测定血清胰岛素和肝脏视黄醇浓度。久坐不动和经过体育训练的大鼠组的每日采食量和每日体重增加相似。中等水平的口服维生素A补充（最高补充剂量约为上述推荐的美国国家研究委员会水平的6倍）并未导致这些畜牧学参数发生任何变化。口服补充维生素A导致肝脏中视黄醇浓度升高（F = 15.2，p < 0.001），但训练和未训练动物之间无显著差异。大鼠的体育训练导致血清胰岛素浓度在统计学上显著降低（1.53±0.18对1.73±0.20）。这种差异非常显著（F = 11.1，p < 0.001）。发现补充维生素A不影响这种负责体内能量代谢调节的激素的浓度。根据估计参数，未证明在体育训练的受试者中过量使用维生素A的必要性。
