Krutkiewicz A, Sałamaszyńska-Guz A, Rzewuska M, Klimuszko D, Binek M
Division of Bacteriology and Molecular Biology, Department of Pre-Clinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw.
Pol J Vet Sci. 2009;12(4):465-72.
A total of 69 Campylobacter jejuni and 16 Campylobacter coli strains isolated from chicken, dog and pig stool samples were characterized based on their resistance to five antimicrobial agents and on plasmid pTet profiles. Antimicrobials used in this study were: amoxicillin/clavulanic acid, ciprofloxacin, erythromycin, tetracycline and trimethoprim/sulfamethoxazole. Among the isolates studied, 91.7% were resistant to one or more antimicrobial agent. The highest level of resistance for the whole test group was to trimethoprim/sulfamethoxazole (57.6%), followed by ciprofloxacin (44.2%) and tetracycline (20%). All isolates were susceptible to amoxicillin/clavulanic acid. Strains isolated from chickens were susceptible to erythromycin. Few erythromycin-resistant strains were isolated from dogs and pigs (5.8%). C. coli strains exhibited a higher antibiotic resistance than C. jejuni strains, excluding resistance to trimethoprim/sulfamethoxazole. The pTet plasmid harboring the tet(O) gene was detected in 14 Campylobacter spp. strains. Our studies demonstrate that the majority (71.4%) of tetracycline-resistant isolates carry a plasmid-borne tet(O) gene, particularly strains for which the minimum inhibitory concentration (MIC) are > or = 256 microg/ml. In conclusion, we have found high-level trimethoprim/sulfamethoxazole, ciprofloxacin and tetracycline resistance in Polish strains isolated from different sources. This study has demonstrated that resistance of Campylobacter species differs depending on both the bacterial species and animal origins. All strains that displayed resistance to four antimicrobial agents were isolated from pigs. Localization of the tet(O) gene on either plasmid or chromosome was not found to be correlated with tetracycline resistance.
从鸡、狗和猪的粪便样本中分离出69株空肠弯曲菌和16株结肠弯曲菌，基于它们对五种抗菌药物的耐药性以及质粒pTet图谱进行特征分析。本研究中使用的抗菌药物为：阿莫西林/克拉维酸、环丙沙星、红霉素、四环素和甲氧苄啶/磺胺甲恶唑。在所研究的分离株中，91.7%对一种或多种抗菌药物耐药。整个测试组中耐药水平最高的是甲氧苄啶/磺胺甲恶唑（57.6%），其次是环丙沙星（44.2%）和四环素（20%）。所有分离株对阿莫西林/克拉维酸敏感。从鸡中分离出的菌株对红霉素敏感。从狗和猪中分离出的耐红霉素菌株很少（5.8%）。除对甲氧苄啶/磺胺甲恶唑的耐药性外，结肠弯曲菌菌株比空肠弯曲菌菌株表现出更高的抗生素耐药性。在14株弯曲菌属菌株中检测到携带tet(O)基因的pTet质粒。我们的研究表明，大多数（71.4%）耐四环素分离株携带质粒介导的tet(O)基因，特别是那些最低抑菌浓度（MIC）≥256μg/ml的菌株。总之，我们在从不同来源分离的波兰菌株中发现了高水平的甲氧苄啶/磺胺甲恶唑、环丙沙星和四环素耐药性。本研究表明，弯曲菌属的耐药性因细菌种类和动物来源而异。所有对四种抗菌药物耐药的菌株均从猪中分离得到。未发现tet(O)基因在质粒或染色体上的定位与四环素耐药性相关。
