Madej J P, Nowacki W, Boratyński J, Borkowski J, Włodarczyk-Szydłowska A, Musiał E
Department of Veterinary Prevention and Immunology, Department and Clinic of Obstetrics, Ruminant Diseases and Animal Health Care, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, Poland.
Pol J Vet Sci. 2009;12(4):499-507.
Vitamin D-binding protein (DBP) participates in the actin scavenger system, it is a carrier of vitamin D and its derivatives, it manifests the capacity to bind mainly monounsaturated and saturated fatty acids, it binds to the surface of several cells and enhances chemotactic activity of C5a of the complement. The present study was aimed at answering the question whether serum DBP level in mares is related to levels of this protein in colostrum and in serum of its progeny. For this purpose, sera from 77 mares, colostra from 72 mares and sera from 69 Thoroughbred foals were collected. Mother's age, number of deliveries experienced in the past, month of delivery, feeding of foals with colostra were recorded. Blood of the foals was sampled from the umbilical vein during delivery (0h) and 36-48 h after delivery from the external jugular vein, colostra of the mares were obtained after delivery and blood of the mares was sampled 36-48 h after delivery. Concentration of DBP was estimated by a self-designed ELISA. In the present study, DBP concentrations in newborn's serum were found independent of their concentrations in mother's serum, her age and number of parities experienced in the past. Colostrum DBP level was found to be lower than that in the mare's serum and was not correlated to the concentration of this protein in mare's serum. There was no effect of colostrum feeding on DBP level in the foal serum. These results indicate that serum DBP concentration in newborn foals depends on factors which act directly on the foal. Because of the lack of correlation between plasma and colostrum concentrations of DBP, it can be assumed that DBP is synthesised in the mammary gland and/or specific transport mechanisms exist in the mammary gland.
维生素 D 结合蛋白（DBP）参与肌动蛋白清除系统，它是维生素 D 及其衍生物的载体，具有主要结合单不饱和脂肪酸和饱和脂肪酸的能力，能与多种细胞表面结合并增强补体 C5a 的趋化活性。本研究旨在回答母马血清 DBP 水平是否与其初乳及后代血清中该蛋白水平相关的问题。为此，采集了 77 匹母马的血清、72 匹母马的初乳以及 69 匹纯种马驹的血清。记录了母马的年龄、既往分娩次数、分娩月份、驹用初乳喂养情况。驹的血液在分娩时（0 小时）从脐静脉采集，分娩后 36 - 48 小时从颈外静脉采集；母马的初乳在分娩后获取，母马的血液在分娩后 36 - 48 小时采集。采用自行设计的 ELISA 法测定 DBP 浓度。在本研究中，发现新生驹血清中 DBP 浓度与其母马血清中的浓度、母马年龄及既往产次无关。初乳中 DBP 水平低于母马血清中的水平，且与母马血清中该蛋白浓度无关。初乳喂养对驹血清中 DBP 水平无影响。这些结果表明，新生驹血清中 DBP 浓度取决于直接作用于驹的因素。由于血浆和初乳中 DBP 浓度缺乏相关性，可推测 DBP 是在乳腺中合成的，和/或乳腺中存在特定的转运机制。
