Wu Genfu, Liu Jie, Ye Zhaoxia
College of Life Sciences, Zhejiang University, Hangzhou 310058 China.
Mikrobiologiia. 2009 Nov-Dec;78(6):809-15.
Phosphobacteria are able to enhance phosphorus availability in soil and improve crop yields. To develop such biofertilizers, 14 predominant phosphobacteria were isolated from eutrophic aquatic ecosystems. Molecular identification and phylogenetic analysis revealed three groups among the nine isolates of inorganic phosphate-solubilizing bacteria (IPSB): IPSB1 and IPSB2 belonged to the actinobacteria and flavobacteria, respectively, and the other seven belonged to the gamma-proteobacteria. Among five isolates of organic phosphorus-mineralizing bacteria (OPMB), two groups were present: OPMB1 and OPMB3 belonged to the beta-proteobacteria, while the other three belonged to the gamma-proteobacteria. The IPSB isolates released 62.8-66.7 mg P 1(-1) from tricalcium phosphate under shaking conditions, and 26.8 to 43.7 mg P 1(-1) under static conditions; the OPMB strains released 23.5-30.2 mg P 1(-1) from lecithin under shaking conditions, and 16.7-27.6 mg P 1(-1) under static conditions. To the best of our knowledge, this is the first report indicating that IPSBI (designated Aureobacterium resistents) as a tricalcium phosphate-solubilizing bacterium and OPMB1 and OPMB3 (designated Acidovorax temperans and Achromobacter xylosoxidans, respectively) are lecithin-mineralizing bacteria. This investigation demonstrated that a eutrophic aquatic ecosystem is a selective source of phosphobacteria and the screened phosphobacteria are a potential alternative to the development of biofertilizers.
磷细菌能够提高土壤中磷的有效性并提高作物产量。为了开发此类生物肥料，从富营养化水生生态系统中分离出了14种优势磷细菌。分子鉴定和系统发育分析表明，在9株无机磷溶解细菌（IPSB）中可分为三组：IPSB1和IPSB2分别属于放线菌和黄杆菌，另外7株属于γ-变形菌。在5株有机磷矿化细菌（OPMB）中，存在两组：OPMB1和OPMB3属于β-变形菌，另外3株属于γ-变形菌。IPSB分离株在振荡条件下从磷酸三钙中释放出62.8 - 66.7 mg P l(-1)，在静态条件下释放出26.8至43.7 mg P l(-1)；OPMB菌株在振荡条件下从卵磷脂中释放出23.5 - 30.2 mg P l(-1)，在静态条件下释放出16.7 - 27.6 mg P l(-1)。据我们所知，这是第一份表明IPSB1（命名为抗金色杆菌）是磷酸三钙溶解细菌以及OPMB1和OPMB3（分别命名为温和嗜酸菌和木糖氧化无色杆菌）是卵磷脂矿化细菌的报告。这项研究表明，富营养化水生生态系统是磷细菌的一个选择性来源，筛选出的磷细菌是开发生物肥料的一种潜在替代品。
