Zuo Li-ping, Liang Yan-bing, Tang Hao, Zhan Hong, Chen Zhi-bin, Li Zhen-yu, Ma Zhong-fu
Department of General Internal Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong, China (ZUO Li-ping works at Renkang Hospital of Nanfang Medical University, Dongguan 523952, Guangdong, China).
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Feb;22(2):93-6.
To investigate the changes and its clinical significance of the expressions of the mRNA and protein of heat shock protein 70 (HSP70) and heme oxygenase-1 (HO-1) genes in the myocardium of acute myocardial infarction (AMI) areas in patients who died suddenly due to AMI.
Specimens of myocardial tissue at infarct site was obtained during autopsy from 18 patients who died suddenly due to AMI, and they were enlisted as study group, and that of myocardial tissue from 17 normal hearts of patients died rapidly after accidents were as control group. The levels of mRNA expression of HSP70 and HO-1 genes were measured in all the specimens by reverse transcription-polymerase chain reaction (RT-PCR) using cDNA samples, and the levels and locations of HSP70 and HO-1 protein expressions in myocardial cells of all specimens were examined by immunohistochemistry (IHC), and the pathological changes in the myocardial tissue were observed.
The expressions of HSP70 mRNA (0.841+/-0.058), HO-1 mRNA (0.918+/-0.161) and HSP70 protein (3 556.68+/-574.19), HO-1 protein (4 336.68+/-865.30) in the myocardium of the AMI areas in the study group were significantly higher than those in control group (the expressions of HSP70 mRNA: 0.105+/-0.034, HO-1 mRNA: 0.086+/-0.053, HSP70 protein: 289.21+/-68.51, HO-1 protein: 1 556.78+/-506.26, all P<0.01), and a weak expression of HSP70 and HO-1 protein was found in the cardiocytes of the AMI area in study group. In the control group, HSP70 protein expression was negative in the cardiocytes, and there was a weak expression of HO-1 protein in the cardiocytes. There was a significant positive correlation between the expressions of mRNA and protein of HSP70 and HO-1 genes at the cardiocytes of patients died suddenly of AMI in study group (r(1)=0.865, r(2)=0.816, both P<0.01).
HSP70 and HO-1 probably were both involved in the pathological and physiological processes of AMI, while HO-1 may express in cardiocytes, but it is more abundant in the cardiocytes in the AMI area.
探讨急性心肌梗死（AMI）猝死患者梗死心肌区域热休克蛋白70（HSP70）和血红素加氧酶-1（HO-1）基因mRNA及蛋白表达的变化及其临床意义。
选取18例因AMI猝死患者尸检时梗死部位心肌组织标本作为研究组，17例因意外事故后迅速死亡患者正常心脏的心肌组织标本作为对照组。采用逆转录-聚合酶链反应（RT-PCR）法，以cDNA样本检测所有标本中HSP70和HO-1基因mRNA表达水平，采用免疫组织化学（IHC）法检测所有标本心肌细胞中HSP70和HO-1蛋白表达水平及定位，并观察心肌组织病理变化。
研究组AMI区域心肌中HSP70 mRNA（0.841±0.058）、HO-1 mRNA（0.918±0.161）及HSP70蛋白（3 556.68±574.19）、HO-1蛋白（4 336.68±865.30）表达均显著高于对照组（HSP70 mRNA表达：0.105±0.034，HO-1 mRNA表达：0.086±0.053，HSP70蛋白：289.21±68.51，HO-1蛋白：1 556.78±506.26，均P<0.01），研究组AMI区域心肌细胞中HSP70和HO-1蛋白呈弱表达。对照组心肌细胞中HSP70蛋白表达为阴性，HO-1蛋白呈弱表达。研究组因AMI猝死患者心肌细胞中HSP70和HO-1基因mRNA与蛋白表达呈显著正相关（r(1)=0.865，r(2)=0.816，均P<0.01）。
HSP70和HO-1可能均参与了AMI的病理生理过程，HO-1可能在心肌细胞中表达，在AMI区域心肌细胞中表达更丰富。
