Dybdahl B, Slørdahl S A, Waage A, Kierulf P, Espevik T, Sundan A
Institute of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway.
Heart. 2005 Mar;91(3):299-304. doi: 10.1136/hrt.2003.028092.
To test the hypothesis that heat shock protein (Hsp) 70 may be released into the circulation after acute myocardial infarction (AMI) by exploring the kinetics of Hsp70 release and the relations between Hsp70 and markers of inflammation and myocardial damage in AMI.
Blood samples from 24 patients were prospectively collected through to the first day after AMI. Hsp70, interleukin (IL) 6, IL-8, and IL-10 in serum were measured by enzyme linked immunosorbent assay (ELISA).
Median Hsp70 concentrations in AMI patients measured at arrival, six hours thereafter, and the following morning were 686, 868, and 607 pg/ml, respectively. These concentrations were all significantly different from those of the control patients with angina with a median serum Hsp70 concentration of 306 pg/ml. Peak Hsp70 correlated with creatine kinase (CK) MB (r = 0.62, p < 0.01) and cardiac troponin T (r = 0.58, p < 0.01). Furthermore, serum Hsp70 correlated with IL-6 and IL-8 at six hours (r = 0.60, p < 0.01 and r = 0.59, p < 0.01, respectively).
In this study, Hsp70 was rapidly released into the circulation after AMI. Circulating Hsp70 is suggested as a marker of myocardial damage. In addition, Hsp70 may have a role in the inflammatory response after AMI.
通过探究热休克蛋白（Hsp）70释放的动力学以及Hsp70与急性心肌梗死（AMI）炎症和心肌损伤标志物之间的关系，来验证急性心肌梗死后Hsp70可能释放到循环系统中的假说。
前瞻性收集24例患者至急性心肌梗死后第一天的血样。采用酶联免疫吸附测定法（ELISA）检测血清中的Hsp70、白细胞介素（IL）-6、IL-8和IL-10。
急性心肌梗死患者入院时、此后6小时及次日早晨测得的Hsp70浓度中位数分别为686、868和607 pg/ml。这些浓度与血清Hsp70浓度中位数为306 pg/ml的心绞痛对照患者均有显著差异。Hsp70峰值与肌酸激酶（CK）同工酶MB（r = 0.62，p < 0.01）和心肌肌钙蛋白T（r = 0.58，p < 0.01）相关。此外，血清Hsp70在6小时时与IL-6和IL-8相关（分别为r = 0.60，p < 0.01和r = 0.59，p < 0.01）。
在本研究中，急性心肌梗死后Hsp70迅速释放到循环系统中。循环中的Hsp70被认为是心肌损伤的标志物。此外，Hsp70可能在急性心肌梗死后的炎症反应中起作用。
