Wadsworth Center, NYS Department of Health, Albany, NY 12201, USA.
Dev Biol. 2010 Apr 15;340(2):480-9. doi: 10.1016/j.ydbio.2010.02.003. Epub 2010 Feb 17.
Angiogenesis, the formation of new blood vessels from existing vasculature, is a complex process that is essential for normal embryonic development. Current models for experimental evaluation of angiogenesis often use tissue from large vessels like the aorta and umbilical vein, which are phenotypically distinct from microvasculature. We demonstrate that the utilization of skin to measure microvascular angiogenesis in embryonic and adult tissues is an efficient way to quantify microvasculature angiogenesis. We validate this approach and demonstrate its added value by showing significant differences in angiogenesis in monogenic and polygenic mouse models. We discovered that the pattern of angiogenic response among inbred mouse strains in this ex vivo assay differs from the strain distributions of previous in vivo angiogenesis assays. The difference between the ex vivo and in vivo assays may be related to systemic factors present in whole animals. Expression analysis of cultured skin biopsies from strains of mice with opposing angiogenic response was performed to identify pathways that contribute to differential angiogenic response. Increased expression of negative regulators of angiogenesis in C57Bl/6J mice was associated with lower growth rates.
血管生成，即从现有脉管系统形成新的血管，是一个复杂的过程，对于正常的胚胎发育至关重要。目前用于血管生成实验评估的模型通常使用来自主动脉和脐静脉等大血管的组织，这些组织在表型上与微血管明显不同。我们证明，利用皮肤来测量胚胎和成年组织中的微血管血管生成是一种量化微血管血管生成的有效方法。我们通过显示单基因和多基因小鼠模型中血管生成的显著差异来验证这种方法及其附加值。我们发现，在这种离体测定中，近交系小鼠之间的血管生成反应模式与以前体内血管生成测定中的菌株分布不同。离体和体内测定之间的差异可能与全身动物中存在的系统性因素有关。对具有相反血管生成反应的小鼠品系的培养皮肤活检进行表达分析，以确定导致差异血管生成反应的途径。C57Bl/6J 小鼠中血管生成负调控因子的表达增加与较低的生长速度有关。
