Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan 410078, People's Republic of China.
Biochem Biophys Res Commun. 2010 Mar 19;393(4):860-3. doi: 10.1016/j.bbrc.2010.02.099. Epub 2010 Feb 18.
WD40 repeat proteins have a wide range of diverse biological functions including signal transduction, cell cycle regulation, RNA splicing, and transcription. Myocardial ischemic preconditioning up-regulated protein 2 (MIP2) is a novel member of the WD40 repeat proteins superfamily that contains five WD40 repeats. Little is known about its biological role, and the purpose of this study was to determine the role of MIP2 in regulating cellular proliferation. Transfection and constitutive expression of MIP2 in the rat cardiomyoblast cell line H9c2 results in enhanced growth of those cells as measured by cell number and is proportional to the amount of MIP2 expressed. Overexpression of MIP2 results in a shorter cell cycle, as measured by flow cytometry. Collectively, these data suggest that MIP2 may participate in the progression of cell proliferation in H9c2 cells.
WD40 重复蛋白具有广泛的生物学功能，包括信号转导、细胞周期调控、RNA 剪接和转录。心肌缺血预处理上调蛋白 2（MIP2）是 WD40 重复蛋白超家族的一个新成员，包含五个 WD40 重复。目前对其生物学作用知之甚少，本研究旨在确定 MIP2 在调节细胞增殖中的作用。MIP2 在大鼠心肌细胞系 H9c2 中的转染和组成型表达导致细胞数量增加，从而增强细胞生长，并且与表达的 MIP2 量成正比。通过流式细胞术测量，MIP2 的过表达导致细胞周期缩短。总之，这些数据表明 MIP2 可能参与 H9c2 细胞的细胞增殖进程。
