Centre for Environmental Contaminants Research, CSIRO Land and Water, PMB 2, Glen Osmond 5064, Australia.
Gen Comp Endocrinol. 2010 May 1;166(3):529-36. doi: 10.1016/j.ygcen.2010.02.015. Epub 2010 Feb 18.
Estrogens are steroidal hormones that control many physiological processes in both female and male vertebrates. Like other vertebrates, fish have two distinct estrogen receptors (Esr) subtypes, Esr1 and Esr2a that have been isolated in a number of species, as well as a third subtype, Esr2b. The mRNA encoding the Esr1 was isolated from the female liver of an Australian freshwater fish, the Murray rainbowfish, Melanotaenia fluviatilis. The rainbowfish esr1 cDNA was 2569 bp in length and with an open reading frame to encode a protein of 611 amino acids. Phylogenetic analysis and multiple amino acid sequence alignment indicated close relationship and high similarity with killifish (Fundulus heteroclitus) and gilthead sea bream (Sparus aurata). Expression of rainbowfish esr1 mRNA was abundant in the liver, gonads and intestine of adult female and male rainbowfish. This is the first isolation of the full-length nucleotide sequence of an estrogen receptor from rainbowfish. This sequence provides a valuable molecular tool that can be used in future studies investigating estrogen mechanisms, actions and tissue-specific expression in juvenile and adult rainbowfish.
雌激素是甾体激素，控制着雌性和雄性脊椎动物的许多生理过程。与其他脊椎动物一样，鱼类有两种不同的雌激素受体（Esr）亚型，Esr1 和 Esr2a，这两种亚型已在许多物种中被分离出来，还有第三种亚型 Esr2b。编码 Esr1 的 mRNA 已从澳大利亚淡水鱼墨瑞鳕鱼（Melanotaenia fluviatilis）的雌鱼肝脏中分离出来。彩虹鱼 esr1 cDNA 长 2569bp，开放阅读框编码 611 个氨基酸的蛋白质。系统发育分析和多种氨基酸序列比对表明，它与食蚊鱼（Fundulus heteroclitus）和金头鲷（Sparus aurata）密切相关，具有高度相似性。成年雌鱼和雄鱼的肝脏、性腺和肠道中均大量表达彩虹鱼 esr1 mRNA。这是首次从彩虹鱼中分离出全长的雌激素受体核苷酸序列。该序列为未来研究幼鱼和成年彩虹鱼的雌激素机制、作用和组织特异性表达提供了有价值的分子工具。
