Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branisovska 31, 37005 Ceske Budejovice, Czech Republic.
Vaccine. 2010 Apr 9;28(17):2993-8. doi: 10.1016/j.vaccine.2010.02.008. Epub 2010 Feb 18.
Ixodes ricinus is one the most abundant tick species in Europe and these ticks transmit pathogens causing human and animal diseases. The cattle ticks, Rhipicephalus (Boophilus) spp., affect cattle production in tropical and subtropical regions of the world. Development of vaccines directed against tick proteins may reduce tick infestations and the transmission of tick-borne pathogens. However, a limiting step in tick vaccine development has been the identification of tick protective antigens. Herein, the tick iron metabolism pathway was targeted in an effort to identify new tick protective antigens. Recombinant I. ricinus (IrFER2) and Rhipicephalus microplus (RmFER2) ferritin 2 proteins were expressed in Escherichia coli and used to immunize rabbits and cattle, respectively. Vaccination with IrFER2 reduced I. ricinus tick numbers, weight and fertility in rabbits with an overall vaccine efficacy (E) of 98%. Control of cattle tick, R. microplus and Rhipicephalus annulatus infestations was obtained in vaccinated cattle with overall E of 64% and 72%, respectively. Notably, the efficacy of the RmFER2 vaccine was similar to that obtained with Bm86 against R. microplus. These collective results demonstrated the feasibility of using ferritin 2 to develop vaccines for the control of tick infestations.
硬蜱属（Ixodes）是欧洲最丰富的蜱种之一，这些蜱传播引起人类和动物疾病的病原体。牛蜱，如璃眼蜱属（Rhipicephalus）（Boophilus） spp.，影响世界热带和亚热带地区的牛生产。针对蜱蛋白的疫苗的开发可能会减少蜱的滋生和蜱传播病原体的传播。然而，蜱疫苗开发的一个限制步骤一直是鉴定蜱保护性抗原。在此，针对蜱铁代谢途径进行了研究，以鉴定新的蜱保护性抗原。重组硬蜱属（IrFER2）和璃眼蜱属（RmFER2）铁蛋白 2 蛋白在大肠杆菌中表达，并分别用于免疫兔和牛。用 IrFER2 免疫接种可减少兔体内的硬蜱属蜱数量、体重和繁殖力，总疫苗效力（E）为 98%。在接种疫苗的牛中，可控制牛蜱、璃眼蜱属（Rhipicephalus）和血红扇头蜱的滋生，总 E 分别为 64%和 72%。值得注意的是，RmFER2 疫苗的功效与 Bm86 对 R. microplus 的功效相似。这些综合结果表明，使用铁蛋白 2 开发疫苗来控制蜱滋生是可行的。
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