Department of Electronics, Computer Sciences and Systems, Bologna University, Italy.
Comput Med Imaging Graph. 2010 Jul;34(5):394-403. doi: 10.1016/j.compmedimag.2010.01.003. Epub 2010 Feb 19.
Laser scanning microscopy provides high-resolution nondestructive in vivo imaging to capture specific structures that have been fluorescently labeled, such as cellular nuclei and membranes, throughout early zebrafish embryogenesis. An increasingly challenging problem biologists must face is how to effectively explore, follow, and study the thousands of cells contained in the resulting time-varying volume data that are large in space, time, and variable domain. Visual data explorations, such as direct volume rendering, have been successfully used for the analysis of volumetric data. However, visualizing large-scale time-varying fields remains a challenging problem. In this paper we present a novel Focus+Context animated volume rendering. The technique is based on the distance map of objects of interest and on a scene graph architecture. We demonstrate that distance map driven volume rendering, implemented in modern graphics hardware, is suited to generate run time and interactive representations such as ghosted rendering and cut-away rendering. The experimental results on zebrafish embryogenesis data demonstrate that the technique is suited to uncover and to analyze biological events, such as organogenesis, contained in time-varying volumetric dataset.
激光扫描显微镜提供高分辨率的非破坏性体内成像，以捕获经过荧光标记的特定结构，如细胞核和膜，贯穿早期斑马鱼胚胎发生。生物学家必须面对的一个日益具有挑战性的问题是如何有效地探索、跟踪和研究在空间、时间和可变域中都很大的时变体积数据中包含的数千个细胞。可视化数据探索，如直接体绘制，已成功用于体积数据的分析。然而，可视化大规模时变场仍然是一个具有挑战性的问题。在本文中，我们提出了一种新颖的聚焦+上下文动画体绘制方法。该技术基于感兴趣对象的距离图和场景图架构。我们证明了基于距离图的体绘制，在现代图形硬件中实现，适合生成运行时和交互表示，如幽灵渲染和切割渲染。在斑马鱼胚胎发生数据上的实验结果表明，该技术适合揭示和分析时变体积数据集所包含的生物事件，如器官发生。
