Akiyama Tomonori, Miyamoto Hiroshi, Fukuda Kazumasa, Sano Naoto, Katagiri Nanako, Shobuike Takeo, Kukita Akiko, Yamashita Yoshio, Taniguchi Hatsumi, Goto Masaaki
Department of Oral and Maxillofacial Surgery, Saga University, Saga, Japan.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 May;109(5):669-76. doi: 10.1016/j.tripleo.2009.10.045. Epub 2010 Feb 19.
The objective of this study was to develop a novel polymerase chain reaction (PCR) method to comprehensively analyze salivary bacterial flora.
The bacterial flora in the saliva of 10 healthy persons and 11 patients with odontogenic infections were examined using a DNA extraction method with a high level of cell destruction efficiency and a novel universal primer set to amplify approximately 580 bp of the 16S rDNA sequence.
Streptococcus (54.5%), Neisseria (14.7%), Actinomyces (8.4%), Gemella (4.1%), Granulicatella (3.8%), and Prevotella (1.4%) were dominant in a total of 1655 clones examined from the saliva of the healthy subjects. The dominant genera differed among the patients with odontogenic infections (a total of 823 clones) and were entirely different from those of the healthy subjects.
This novel comprehensive salivary bacterial flora analysis method may be a useful supportive method to identify causative agents of odontogenic infections.
本研究的目的是开发一种新型聚合酶链反应（PCR）方法，以全面分析唾液细菌菌群。
使用具有高细胞破坏效率的DNA提取方法和一种新型通用引物组，对10名健康人和11名牙源性感染患者的唾液中的细菌菌群进行检测，以扩增约580 bp的16S rDNA序列。
在从健康受试者唾液中检测的总共1655个克隆中，链球菌（54.5%）、奈瑟菌（14.7%）、放线菌（8.4%）、孪生球菌（4.1%）、颗粒链菌属（3.8%）和普雷沃菌属（1.4%）占主导地位。牙源性感染患者（总共823个克隆）中的优势菌属有所不同，且与健康受试者的完全不同。
这种新型的全面唾液细菌菌群分析方法可能是一种有用的辅助方法，用于识别牙源性感染的病原体。
