大剂量抗坏血酸诱导间皮瘤细胞死亡。

High dose of ascorbic acid induces cell death in mesothelioma cells.

机构信息

Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.

出版信息

Biochem Biophys Res Commun. 2010 Apr 2;394(2):249-53. doi: 10.1016/j.bbrc.2010.02.012. Epub 2010 Feb 19.

DOI:10.1016/j.bbrc.2010.02.012

PMID:20171954

Abstract

Malignant mesothelioma is an asbestos-related fatal disease with no effective cure. Recently, high dose of ascorbate in cancer treatment has been reexamined. We studied whether high dose of ascorbic acid induced cell death of four human mesothelioma cell lines. High dose of ascorbic acid induced cell death of all mesothelioma cell lines in a dose-dependent manner. We further clarified the cell killing mechanism that ascorbic acid induced reactive oxygen species and impaired mitochondrial membrane potential. In vivo experiment, intravenous administration of ascorbic acid significantly decreased the growth rate of mesothelioma tumor inoculated in mice. These data suggest that ascorbic acid may have benefits for patients with mesothelioma.

摘要

恶性间皮瘤是一种与石棉有关的致命疾病，目前尚无有效治疗方法。最近，人们重新审视了大剂量维生素 C 在癌症治疗中的作用。本研究旨在探讨高浓度抗坏血酸是否诱导四种人恶性间皮瘤细胞系发生细胞死亡。结果显示，高浓度抗坏血酸以剂量依赖性方式诱导所有间皮瘤细胞系发生细胞死亡。本研究进一步阐明了抗坏血酸诱导活性氧产生和破坏线粒体膜电位从而导致细胞死亡的机制。在体内实验中，静脉给予抗坏血酸可显著降低接种于小鼠的间皮瘤肿瘤的生长速度。这些数据表明，抗坏血酸可能对间皮瘤患者有益。