Laboratory Medicine, Immunology, University Hospitals Leuven, Catholic University of Leuven, Leuven, Belgium.
Clin Chim Acta. 2010 Jul 4;411(13-14):931-5. doi: 10.1016/j.cca.2010.02.060. Epub 2010 Feb 19.
BACKGROUND: Detection of IgG antibodies against deamidated gliadin peptides (DGP) is more sensitive and more specific for celiac disease than detection of IgG antibodies against native gliadin. Our aim was to evaluate the technical performance and diagnostic accuracy of four commercial IgG anti-DGP assays. METHODS: Commercial IgG anti-DGP assays from Euroimmun, Inova, Phadia and The Binding Site were evaluated and their diagnostic accuracy (sensitivity and specificity) compared to other serologic assays for celiac disease (3IgA and 2IgG anti-tTG assays, 1IgA and 1IgG anti-gliadin assay, 1IgA anti-DGP assay). The study population consisted of 86 consecutive CD patients and 741 disease controls. RESULTS: The technical performance (linearity, interference and imprecision) of the IgG anti-DGP assays was acceptable. The sensitivity of the IgG anti-DGP assays varied between 76.7% and 86.0% at the cut-off recommended by the manufacturer and between 74.4% and 86.0% at the cut-off that corresponded to a specificity of 98%. The specificity varied between 97.3% and 99.3%. The diagnostic accuracy of the IgG anti-DGP assays was comparable to the diagnostic accuracy of the IgA anti-tTG assays. The sensitivity of the IgG anti-DGP assays was significantly better than sensitivity of the IgG anti-tTG assays (p<0.05) and the specificity was significantly better than the IgA and IgG anti-gliadin assays (p<0.05). CONCLUSIONS: The overall performance of the four IgG anti-DGP assays was acceptable and the diagnostic accuracy comparable to the three IgA anti-tTG assays.
背景：与检测天然麦醇溶蛋白相比，检测脱酰胺麦醇溶蛋白肽（DGP）的 IgG 抗体对乳糜泻的检测更敏感、更特异。我们的目的是评估四种商业 IgG 抗-DGP 检测方法的技术性能和诊断准确性。
方法：评估了 Euroimmun、Inova、Phadia 和 The Binding Site 的商业 IgG 抗-DGP 检测方法，并将其诊断准确性（敏感性和特异性）与其他乳糜泻血清学检测方法（3 种 IgA 和 2 种 IgG 抗-tTG 检测方法、1 种 IgA 和 1 种 IgG 抗麦醇溶蛋白检测方法、1 种 IgA 抗-DGP 检测方法）进行比较。研究人群包括 86 例连续的 CD 患者和 741 例疾病对照组。
结果：IgG 抗-DGP 检测方法的技术性能（线性、干扰和不精密度）可接受。在制造商推荐的临界值下，IgG 抗-DGP 检测方法的敏感性在 76.7%至 86.0%之间，在与特异性为 98%对应的临界值下，敏感性在 74.4%至 86.0%之间。特异性在 97.3%至 99.3%之间。IgG 抗-DGP 检测方法的诊断准确性与 IgA 抗-tTG 检测方法的诊断准确性相当。IgG 抗-DGP 检测方法的敏感性明显优于 IgG 抗-tTG 检测方法（p<0.05），特异性明显优于 IgA 和 IgG 抗麦醇溶蛋白检测方法（p<0.05）。
结论：四种 IgG 抗-DGP 检测方法的总体性能可接受，诊断准确性与三种 IgA 抗-tTG 检测方法相当。
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