Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
J Immunol Methods. 2010 Apr 15;355(1-2):76-9. doi: 10.1016/j.jim.2010.02.006. Epub 2010 Feb 19.
Western blotting is a powerful technique to characterize a multitude of cellular proteins. As an internal control, the blots are commonly probed for "housekeeping" gene products. In this communication, we show that cell confluence significantly affects the levels of two such widely used proteins, alpha-tubulin and Glyceraldehyde-3-Phosphate Dehydrogenase. On the other hand the levels of heat-shock protein-90 and beta-actin remained unchanged at a wide range of cell densities, making these proteins into more reliable loading controls.
蛋白质印迹法是一种鉴定多种细胞蛋白的强大技术。作为内参，印迹法通常探测“管家”基因产物。在本通讯中，我们表明细胞汇合显著影响两种广泛使用的蛋白质（α-微管蛋白和甘油醛-3-磷酸脱氢酶）的水平。另一方面，热休克蛋白-90 和 β-肌动蛋白的水平在广泛的细胞密度范围内保持不变，使这些蛋白质成为更可靠的加载对照。
