Ramis G, Martínez-Alarcón L, Majado M, Ríos A, Quereda J J, Ramírez P, Muñoz A
Departamento de Producción Animal, Universidad de Murcia, Murcia, Spain.
Transplant Proc. 2010 Jan-Feb;42(1):387-9. doi: 10.1016/j.transproceed.2009.11.012.
To assess the presence of irregular xenoantibodies against human red blood cells (RBCs) in 6 primate species used in xenotransplantation and other experimental procedures.
Serum samples from 109 baboons of 4 different species (olive, chacma, sacred, and Guinea), 38 rhesus macaques, and 30 squirrel monkeys were tested for irregular xenoantibodies using an agglutination test using human RBCs of known phenotype for Rh, Kell, Kidd, Lewis, Lutheran, P1, and Duffy antigens, commercially available as RBC I, II, and III.
We found hemagglutination for RBC I in 49%, 22%, 100%, 57%, 32%, and 33% of olive, chacma, sacred, and Guinea baboons, rhesus macaques, and squirrel monkey, respectively. The frequency for RBC II was 49%, 50%, 100%, 57%, 37%, and 33%, respectively, and for RBC III was 56%, 37%, 100%, 79%, 34%, and 33%, respectively. There were differences in frequency depending on the sex of the rhesus macaques; all 3 RBCs tested were higher in the females: 44% vs 0%, P = .008; 48% vs 1%, P = .02, and 44% vs 9.1%, P = .04 for RBC I, II, and III, respectively. There were differences due to age in only olive baboons, and a higher frequency in younger animals compared with juvenile, subadult, and adult animals for all 3 human RBCs.
Assessment of irregular antibodies in the presence of primate serum should be taken into account during any experimental xenotransplantation protocol.
评估用于异种移植和其他实验程序的6种灵长类动物中针对人类红细胞（RBC）的不规则异种抗体的存在情况。
使用已知Rh、Kell、Kidd、Lewis、Lutheran、P1和Duffy抗原表型的人红细胞，通过凝集试验检测来自4种不同物种（橄榄狒狒、南非大狒狒、神圣狒狒和几内亚狒狒）的109只狒狒、38只恒河猴和30只松鼠猴的血清样本中是否存在不规则异种抗体，这些红细胞以RBC I、II和III的形式商购可得。
我们发现，在橄榄狒狒、南非大狒狒、神圣狒狒和几内亚狒狒、恒河猴和松鼠猴中，分别有49%、22%、100%、57%、32%和33%的样本对RBC I发生血凝反应。对RBC II的反应频率分别为49%、50%、100%、57%、37%和33%，对RBC III的反应频率分别为56%、37%、100%、79%、34%和33%。恒河猴的反应频率因性别而异；检测的所有3种RBC在雌性中的反应频率更高：RBC I分别为44%和0%，P = 0.008；RBC II分别为48%和1%，P = 0.02；RBC III分别为44%和9.1%，P = 0.04。仅在橄榄狒狒中观察到因年龄导致的差异，对于所有3种人红细胞，幼年动物的反应频率高于幼年、亚成年和成年动物。
在任何实验性异种移植方案中，都应考虑对灵长类动物血清中不规则抗体的评估。
