Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
J Plant Physiol. 2010 Jun 15;167(9):709-16. doi: 10.1016/j.jplph.2009.12.015. Epub 2010 Feb 21.
Light-induced chloroplast avoidance movement has long been considered to be a photoprotective mechanism. Here, we present an experimental model in which this function can be shown for wild type Arabidopsis thaliana. We used blue light of different fluence rates for chloroplast positioning, and strong red light inactive in chloroplast positioning as a stressing light. The performance of photosystem II was measured by means of chlorophyll fluorescence. After stressing light treatment, a smaller decrease in photosystem II quantum yield was observed for leaves with chloroplasts in profile position as compared with leaves with chloroplasts in face position. Three Arabidopsis mutants, phot2 (no avoidance response), npq1 (impaired zeaxanhtin accumulation) and stn7 (no state transition), were examined for their chloroplast positioning and chlorophyll fluorescence parameters under identical experimental conditions. The results obtained for these mutants revealed additional stressing effects of blue light as compared with red light.
光诱导的叶绿体避动运动长期以来被认为是一种光保护机制。在这里，我们提出了一个实验模型，在这个模型中，可以观察到野生型拟南芥的这种功能。我们使用不同光强的蓝光进行叶绿体定位，而强红光（对叶绿体定位无作用）作为应激光。通过叶绿素荧光测量光系统 II 的性能。在应激光处理后，与叶绿体处于正面位置的叶片相比，叶绿体处于侧面位置的叶片的光系统 II 量子产量下降幅度较小。在相同的实验条件下，对三种拟南芥突变体（ phot2 （无避动反应）、 npq1 （玉米黄质积累受损）和 stn7 （无状态转换））进行了叶绿体定位和叶绿素荧光参数的检测。与红光相比，这些突变体的结果显示出蓝光的额外应激效应。
