Hygiene Unit, S. Martino Hospital, Genoa, Italy.
J Clin Virol. 2010 Apr;47(4):372-5. doi: 10.1016/j.jcv.2010.01.014. Epub 2010 Feb 20.
Previous studies have shown a high HIV-1 genetic variability in the Republic of Congo. This can greatly influence the performance of molecular assays for HIV-1 diagnosis.
To define a reliable test for detection of HIV-1 DNA in this area.
We compared a commercial nested PCR (C-PCR) assay and an in house nested PCR (H-PCR) assay for the detection of HIV-1 DNA in the first 30 seropositive pregnant women enrolled into the ongoing "Kento-Mwana" project, for the prevention of HIV mother-to-child transmission in the city of Pointe Noire, Republic of Congo, Africa. Sequencing and phylogenetic analysis of partial HIV-1 pol sequences were also performed.
C-PCR detected HIV-1 DNA in only 15/30 samples from seropositive women (50.0%), as opposed to 29/30 (96.6%) by H-PCR (P<0.0001). Phylogenetic analysis and bootscanning showed that only 10 sequences could be assigned to known clades (seven pure subtypes and three circulating recombinant forms), whereas the other 20 sequences were unique recombinant forms.
The great genetic variability of HIV-1 in this area strongly advises to for using molecular methods only after local validation to avoid false negative results.
先前的研究表明，刚果共和国的 HIV-1 遗传变异性很高。这可能会极大地影响 HIV-1 诊断的分子检测结果。
确定在该地区检测 HIV-1 DNA 的可靠方法。
我们比较了商用巢式 PCR（C-PCR）检测法和自行设计的巢式 PCR（H-PCR）检测法，用于检测非洲刚果共和国黑角市“Kento-Mwana”项目中首批 30 名血清阳性孕妇的 HIV-1 DNA。我们还对部分 HIV-1 pol 序列进行了测序和系统进化分析。
C-PCR 仅在 30 名血清阳性妇女中的 15 份样本（50.0%）中检测到 HIV-1 DNA，而 H-PCR 则在 30 份样本中均检测到（96.6%）（P<0.0001）。系统进化分析和 bootscanning 显示，只有 10 个序列可被归为已知的分支（7 个纯亚型和 3 个循环重组形式），而其余 20 个序列为独特的重组形式。
该地区 HIV-1 的遗传高度多样性强烈建议在进行分子检测之前进行本地验证，以避免假阴性结果。
