Bruzzone Bianca, Bisio Francesca, Caligiuri Patrizia, Mboungou Franc A Mayinda, Nigro Nicola, Sticchi Laura, Ventura Agostina, Saladini Francesco, Zazzi Maurizio, Icardi Giancarlo, Viscoli Claudio
Hygiene Unit, IRCCS AOU San Martino-IST, Genoa, Italy.
Infectious Diseases Unit, University of Genoa and IRCCS AOU San Martino-IST, Genoa, Italy.
J Virol Methods. 2014 Jul;203:102-6. doi: 10.1016/j.jviromet.2014.02.030. Epub 2014 Mar 30.
Accurate HIV-1 RNA quantitation is required to support the scale up of antiretroviral therapy in African countries. Extreme HIV-1 genetic variability in Africa may affect the ability of commercially available assays to detect and quantify HIV-1 RNA accurately. The aim of this study was to compare three real-time PCR assays for quantitation of plasma HIV-1 RNA levels in patients from the Republic of Congo, an area with highly diversified HIV-1 subtypes and recombinants. The Abbott RealTime HIV-1, BioMérieux HIV-1 EasyQ test 1.2 and Cobas AmpliPrep/Cobas TaqMan HIV-1 1.0 were compared for quantitation of HIV-1 RNA in 37 HIV-1 seropositive pregnant women enrolled in the Kento-Mwana project for prevention of mother-to-child transmission in Pointe-Noire, Republic of Congo. The sample panel included a variety of HIV-1 subtypes with as many as 21 (56.8%) putative unique recombinant forms. Qualitative detection of HIV-1 RNA was concordant by all three assays in 33/37 (89.2%) samples. Of the remaining 4 (10.8%) samples, all were positive by Roche, three by Abbott and none by BioMérieux. Differences exceeding 1Log in positive samples were found in 4/31 (12.9%), 10/31 (32.3%) and 5/31 (16.1%) cases between Abbott and BioMérieux, Roche and BioMérieux, and Abbott and Roche, respectively. In this sample panel representative of highly polymorphic HIV-1 in Congo, the agreement among the three assays was moderate in terms of HIV-1 RNA detectability and rather inconsistent in terms of quantitation.
准确的HIV-1 RNA定量对于支持非洲国家扩大抗逆转录病毒治疗规模至关重要。非洲地区HIV-1的极端基因变异性可能会影响市售检测方法准确检测和定量HIV-1 RNA的能力。本研究的目的是比较三种实时PCR检测方法，以定量来自刚果共和国患者血浆中的HIV-1 RNA水平，该地区的HIV-1亚型和重组体高度多样化。比较了雅培实时HIV-1检测法、生物梅里埃HIV-1 EasyQ检测法1.2和科巴思AmpliPrep/科巴思TaqMan HIV-1检测法（版本1.0），对37名参与刚果共和国黑角市Kento-Mwana预防母婴传播项目的HIV-1血清阳性孕妇的HIV-1 RNA进行定量。样本组包括多种HIV-1亚型，其中多达21种（56.8%）为假定的独特重组形式。三种检测方法对33/37（89.2%）的样本进行HIV-1 RNA定性检测结果一致。在其余4份（10.8%）样本中，罗氏检测法均呈阳性，雅培检测法有3份呈阳性，生物梅里埃检测法均为阴性。在雅培与生物梅里埃、罗氏与生物梅里埃、雅培与罗氏之间，分别有4/31（12.9%）、10/31（32.3%）和5/31（16.1%）的阳性样本差异超过1个对数。在这个代表刚果高度多态性HIV-1的样本组中，三种检测方法在HIV-1 RNA检测能力方面一致性一般，在定量方面则相当不一致。
