Sanai T, Onoyama K, Osato S, Motomura K, Oochi N, Oh Y, Okuda S, Takaichi S, Fujishima M
Second Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Nephron. 1991;57(3):349-54. doi: 10.1159/000186286.
The dose dependency of germanium dioxide(GeO2)-induced nephrotoxicity was investigated experimentally in rat groups orally treated with high (150 mg/kg/day), moderate (75 mg/kg/day), or low (37.5 mg/kg/day) doses of GeO2, and in an untreated group. Renal dysfunction, indicated by the increase of blood urea nitrogen and the decrease of creatinine clearance, and systemic toxicity by weight loss, anemia, and hypoproteinemia were more apparent in rats treated with higher dose of GeO2. Urinalysis including daily urinary protein excretion did not reveal any abnormalities in any of the groups. Urinary excretion and renal-tissue content of Ge were significantly elevated in the group of the higher dose of GeO2. Light microscopically, vacuolar degeneration and depositions of granules positive for periodic acid-Schiff in distal tubules were predominant in the higher-dose group of GeO2. The present study demonstrates that GeO2-induced nephrotoxicity develops dose dependently.
在分别经口给予高剂量（150毫克/千克/天）、中等剂量（75毫克/千克/天）或低剂量（37.5毫克/千克/天）二氧化锗（GeO₂）的大鼠组以及未处理组中，对GeO₂诱导的肾毒性的剂量依赖性进行了实验研究。高剂量GeO₂处理的大鼠中，以血尿素氮升高和肌酐清除率降低为指标的肾功能障碍以及以体重减轻、贫血和低蛋白血症为表现的全身毒性更为明显。包括每日尿蛋白排泄量在内的尿液分析在任何组中均未发现任何异常。高剂量GeO₂组的Ge尿排泄量和肾组织含量显著升高。在光学显微镜下，高剂量GeO₂组远端肾小管中液泡变性和高碘酸-希夫染色阳性颗粒沉积占主导。本研究表明，GeO₂诱导的肾毒性呈剂量依赖性发展。
