Dalgarno Paul A, Dalgarno Heather I C, Putoud Aurélie, Lambert Robert, Paterson Lynn, Logan David C, Towers David P, Warburton Richard J, Greenaway Alan H
School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, UK.
Opt Express. 2010 Jan 18;18(2):877-84. doi: 10.1364/OE.18.000877.
A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving approximately 10 nm z position resolution by parameterising the images with an image sharpness metric.
传统显微镜仅从单个物平面生成清晰图像。这通常是一个限制，在细胞生物学领域尤为明显。我们展示了一种显微镜附件，它能同时从多个物平面记录清晰图像。关键概念是在光学系统中引入一个扭曲的衍射光栅，建立与级次相关的聚焦能力，以生成多个图像，每个图像都来自不同的物平面。我们不仅将这种多平面成像用于生物成像，还用于纳米颗粒跟踪，通过用图像清晰度指标对图像进行参数化，实现了约10纳米的z位置分辨率。
