有和没有肌醇六磷酸时人血红蛋白的一氧化碳和一氧化氮玻尔效应

The CO and NO Bohr effect of human hemoglobin with and without inositolhexaphosphate.

作者信息

de Bruin S H, Boen F J, Rollema H S, van Beek G G

出版信息

Biophys Chem. 1977 Sep;7(2):169-72. doi: 10.1016/0301-4622(77)80010-0.

PMID:20174

Abstract

Using NO and CO as ligands the Bohr effect of human hemoglobin has been measured with and without inositolhexophosphate. It appears that in the absence and presence of inositolhexaphosphate hemoglobin shows a distinct ligand specificity with respect to the Bohr effect. Ligation with NO is accompanied by release of a larger number of Bohr effect. It is shown that this latter result is due to the fact that the number of protons taken up upon binding of inositolhexaphosphate to ligated hemoglobin is larger for HbNO than for HbCO. It is suggested that this additional proton uptake is partially due to a restoration of the saltbridge between His 146beta and Asp 94beta upon addition of IHP.

摘要

以一氧化氮（NO）和一氧化碳（CO）作为配体，在有和没有肌醇六磷酸的情况下测量了人血红蛋白的玻尔效应。结果表明，无论有无肌醇六磷酸，血红蛋白在玻尔效应方面都表现出明显的配体特异性。与NO结合时伴随着大量玻尔效应的释放。结果表明，后一结果是由于肌醇六磷酸与结合配体的血红蛋白结合时，HbNO比HbCO摄取的质子数更多。有人认为，这种额外的质子摄取部分是由于添加肌醇六磷酸后，His 146β和Asp 94β之间的盐桥得以恢复。