有机磷酸盐对人血红蛋白价态杂种玻尔效应的影响。

The influence of organic phosphates on the Bohr effect of human hemoglobin valency hybrids.

作者信息

Rollema H S, De Bruin S H, Van Os G A

出版信息

Biophys Chem. 1976 May;4(3):223-8. doi: 10.1016/0301-4622(76)80068-3.

Abstract

The Bohr effect of hemoglobin and that of the aquomet and cyanomet valency hybrids was measured in the presence and the absence of IHP (inositol hexaphosphate) and DPG (2,3-diphosphoglycerate). In the absence of these organic phosphates the four hybrids show similar, but suppressed Bohr effects as compared to hemoglobin. Addition of IHP and DPG results in all cases in an increase of the Bohr effect. The additional phosphate induced Bohr effect of the hybrids with the alpha chain in the oxidized form is almost identical to that of hemoglobin, while this effect of the hybrids with oxidized beta chains is slighly lower than that of hemoglobin. The results suggest (a) that the Bohr effect is correlated to the ligation state of the hemoglobin molecule rather than to its quaternary structure (b) that the additional phosphate induced Bohr effect is related to the change in quaternary structure of the tetramer, and (c) that with respect to the Bohr effect of the hybrids there is no difference between high and low spin species.

摘要

在有和没有肌醇六磷酸（IHP）及2,3-二磷酸甘油酸（DPG）的情况下，测定了血红蛋白、高铁血红蛋白和氰化高铁血红蛋白价态杂种的玻尔效应。在没有这些有机磷酸盐的情况下，与血红蛋白相比，这四种杂种表现出相似但受到抑制的玻尔效应。添加IHP和DPG在所有情况下都会导致玻尔效应增加。氧化形式的α链杂种的额外磷酸盐诱导玻尔效应几乎与血红蛋白相同，而氧化β链杂种的这种效应略低于血红蛋白。结果表明：（a）玻尔效应与血红蛋白分子的连接状态相关，而非与其四级结构相关；（b）额外磷酸盐诱导的玻尔效应与四聚体四级结构的变化有关；（c）就杂种的玻尔效应而言，高自旋和低自旋物种之间没有差异。