Maurer Christian, Khan Saranjam, Fassl Stephanie, Bernet Stefan, Ritsch-Marte Monika
Innsbruck Medical University, Division for Biomedical Physics, Müllerstrasse 44, 6020 Innsbruck, Austria.
Opt Express. 2010 Feb 1;18(3):3023-34. doi: 10.1364/OE.18.003023.
We demonstrate "depth of field multiplexing" by a high resolution spatial light modulator (SLM) in a Fourier plane in the imaging path of a standard microscope. This approach provides simultaneous imaging of different focal planes in a sample with only a single camera exposure. The phase mask on the SLM corresponds to a set of superposed multi-focal off-axis Fresnel lenses, which sharply image different focal planes of the object to non-overlapping adjacent sections of the camera chip. Depth of field multiplexing allows to record motion in a three dimensional sample volume in real-time, which is exemplarily demonstrated for cytoplasmic streaming in plant cells and rapidly swimming protozoa.
我们在标准显微镜成像路径的傅里叶平面中，通过高分辨率空间光调制器（SLM）展示了“景深复用”。这种方法仅需一次相机曝光，就能同时对样品的不同焦平面进行成像。SLM上的相位掩模对应于一组叠加的多焦点离轴菲涅耳透镜，这些透镜将物体的不同焦平面清晰地成像到相机芯片的不重叠相邻区域。景深复用能够实时记录三维样品体积中的运动，这在植物细胞的细胞质流动和快速游动的原生动物中得到了示例性证明。
