Greenwald D, Mass D, Gottlieb L, Tuel R
Section of Plastic Surgery, University of Chicago Pritzker School of Medicine, Ill.
Plast Reconstr Surg. 1991 May;87(5):925-30; discussion 931-2. doi: 10.1097/00006534-199105000-00018.
This in vitro study was designed to test the hypothesis that flexor tendons have an intrinsic capability to heal as judged by rigorously defined biomechanical criteria. Rabbit forepaw flexor tendons (n = 114) were transected and repaired in zone II and cultured in standard media or media supplemented with vitamin A or E. Tenorrhaphies were disrupted at 0, 2, 8, and 12 weeks after repair by fixed-speed tensiometry. The following parameters were calculated: true stress, true strain, and normalized energy absorption. Statistical analysis was by Student's t test. Peak stress (strength) increased equally in all groups over time (p less than 0.05), while normalized energy absorption (toughness) was markedly higher in the vitamin E-treated group after 2 weeks' incubation (p less than 0.05). These data represent the first biomechanical evidence of intrinsic tendon healing in vitro. Biomechanical methodology and vitamin effects are discussed in detail.
根据严格定义的生物力学标准判断，屈肌腱具有内在的愈合能力。将兔前爪屈肌腱（n = 114）在II区横断并修复，然后在标准培养基或添加了维生素A或维生素E的培养基中培养。通过固定速度张力测定法在修复后0、2、8和12周破坏肌腱缝合处。计算以下参数：真应力、真应变和标准化能量吸收。采用学生t检验进行统计分析。随着时间推移，所有组的峰值应力（强度）均同等增加（p < 0.05），而在孵育2周后，维生素E处理组的标准化能量吸收（韧性）明显更高（p < 0.05）。这些数据代表了体外肌腱内在愈合的首个生物力学证据。文中详细讨论了生物力学方法和维生素的作用。
