Department of Pharmaceutical and Medicinal Chemistry, School of Pharmacy, Jena, Germany.
J Sep Sci. 2010 Apr;33(8):1085-9. doi: 10.1002/jssc.200900739.
A neutral hydrophobic monolith prepared by radical in situ copolymerization of lauryl methacrylate and ethylene dimethacylate has been evaluated for the CEC separation of diastereomers of small peptides using acidic mobile phases containing ACN as organic modifier. Using an acidic mobile phase, the peptides migrated due to their own electrophoretic mobility. Hydrophobic interactions with the stationary phase contributed to the separation. Peptide mobility and resolution increased with increasing the ACN content. Retention times increased with the pH of the mobile phase. Peak resolution increased with buffer pH and concentration. Di- and tripeptides composed only of L-configured amino acids migrated faster than peptides containing D-amino acids. A mixture of isomeric Asp tripeptides that could not be completely resolved by either CZE or HPLC as well as the 24mer peptides tetracosactide and (16)[D-Lys]-tetracosactide could also be separated by CEC on the hydrophobic monolith.
一种通过甲基丙烯酸十二酯和乙二醇二甲基丙烯酸酯的自由基原位共聚制备的中性疏水整体柱，已被评估用于使用含有 ACN 的酸性流动相作为有机改性剂的手性 CE 分离小肽的非对映异构体。在酸性流动相中，由于其自身的电泳迁移率，肽迁移。与固定相的疏水相互作用有助于分离。随着 ACN 含量的增加，肽的迁移率和分辨率增加。保留时间随流动相的 pH 值增加而增加。峰分辨率随缓冲液 pH 值和浓度的增加而增加。仅由 L-构型氨基酸组成的二肽和三肽的迁移速度比含有 D-氨基酸的肽快。通过 CZE 或 HPLC 均无法完全分离的异构天冬氨酸三肽混合物以及 24 肽四肽和（16）[D-Lys]-四肽也可以通过疏水整体柱上的 CEC 分离。
