Department of Plant Biology, Plant Pathology Section, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej, Frederiksberg C, Denmark.
J Basic Microbiol. 2010 Feb;50(1):104-9. doi: 10.1002/jobm.200900019.
In the Andean region of South America downy mildew, caused by Peronospora farinosa, is the most important disease of quinoa (Chenopodium quinoa). Peronospora farinosa, a highly polyphyletic species, occurs on quinoa and wild relatives on all continents. However, very little is known about the geographic diversity of the pathogen. As the interest in quinoa as a novel crop is increasing worldwide, geographical differences in the population structure of the downy mildew pathogen must be taken into consideration in order to design appropriate control strategies under a variety of circumstances. As a step towards understanding the geographic diversity of P. farinosa from quinoa, 40 downy mildew isolates from the Andean highlands and Denmark were characterized using universally primed PCR (UP-PCR). Eight UP-PCR primers were tested. A combined analysis of markers separated the Danish and Andean isolates in two distinct clusters. This study raises new questions about the origin and spread of P. farinosa on quinoa, its geographic diversity and host specificity.
在南美洲的安第斯地区，由霜霉病菌（Peronospora farinosa）引起的霜霉病是藜麦（Chenopodium quinoa）最重要的病害。霜霉病菌是一个高度多系的物种，存在于藜麦和所有大陆的野生近缘种上。然而，人们对病原体的地理多样性知之甚少。由于藜麦作为一种新型作物在全球范围内的兴趣日益增加，必须考虑到霜霉病病原体的种群结构在地理上的差异，以便在各种情况下制定适当的控制策略。作为了解藜麦霜霉病菌地理多样性的一个步骤，从安第斯高地和丹麦采集了 40 个霜霉病分离株，使用通用引物 PCR（UP-PCR）进行了特征描述。测试了 8 个 UP-PCR 引物。标记物的综合分析将丹麦和安第斯分离株分为两个不同的聚类。这项研究提出了关于霜霉病菌在藜麦上的起源和传播、地理多样性和宿主特异性的新问题。
