定量分析分子紧密连接蛋白 4 的表达改善恶性浆膜腔积液的诊断。

Improvement of malignant serous effusions diagnosis by quantitative analysis of molecular claudin 4 expression.

机构信息

Department of Immunology, University Hospital Center, Saint-Etienne, France.

出版信息

Biomarkers. 2010 Jun;15(4):315-24. doi: 10.3109/13547501003658114.

PMID:20175704

Abstract

Claudin gene expression is frequently altered in human cancers. Our aim was to improve the cytology diagnosis of malignancy in serous fluids with the quantification of claudins compared with various classic molecular markers using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method. Peritoneal or pleural effusions of 56 patients were assessed as malignant from histological analysis and 19 as benign. Claudin 4 was the most significantly upregulated (68%) marker in patients with malignant effusions. In cytologically negative malignant effusions, claudin 4 was found increased in 8/18 fluids. Quantitative RT-PCR is a sensitive method for the detection of free cancer cells in serous effusions.

摘要

Claudin 基因表达在人类癌症中经常发生改变。我们的目的是通过实时逆转录-聚合酶链反应 (RT-PCR) 方法，与各种经典的分子标志物相比，利用 Claudin 的定量来提高对浆膜液恶性肿瘤的细胞学诊断。从组织学分析中评估了 56 名患者的腹膜或胸腔积液为恶性，19 名患者为良性。Claudin 4 是恶性积液中上调最显著的（68%）标志物。在细胞学阴性的恶性积液中，发现 Claudin 4 在 18 种液体中有 8 种增加。定量 RT-PCR 是检测浆膜液中游离癌细胞的敏感方法。