Department of Anatomy, Cell Biology, Physiology and Biophysics, Institute of Biology, State University of Campinas, Campinas, SP, Brazil.
Connect Tissue Res. 2010 Aug;51(4):265-73. doi: 10.3109/03008200903318279.
The aim of this study was to evaluate if spontaneous (nonforced active) exercise and age (maturation process) alter the biomechanical and biochemical properties of superficial digital flexor tendon. Chickens aged 1, 5, and 8 months were divided into two groups: caged and penned. The caged group was reared in an area of 0.5 m(2) (3 animals/cage), while the penned group was reared in an area of 60 m(2) (3 animals/area). For biochemical analysis, the tendon was divided into tensile and compressive regions for quantification of hydroxyproline and glycosaminoglycan content. Biomechanical properties were analyzed from tensile tests of intact tendons. The biomechanical measurements were taken at maximum load and maximum stress. In both the caged and penned groups, maximum load and energy absorption increased with maturation; however, the elastic modulus, maximum stress, and maximum strain did not increase with maturation. Exercise resulted in a higher load, stress, and elastic modulus in the fifth month. Collagen content increased with age in the penned group and with exercise in the fifth and eighth months. Exercise results in a higher expression of glycosaminoglycans in young tendons compared to mature tendons. Thus, low-intensity mechanical stimuli promote the synthesis and possible rearrangement of molecules in immature tendons, whereas inactivity leads to deleterious effects on the material properties (maximum stress and elastic modulus) during growth and maturation.
本研究旨在评估自发性(非强制主动)运动和年龄(成熟过程)是否会改变浅层屈趾肌腱的生物力学和生化特性。1、5 和 8 月龄的鸡分为两组:笼养组和围栏组。笼养组饲养在 0.5 m²(每笼 3 只)的区域,围栏组饲养在 60 m²(每区 3 只)的区域。为了进行生化分析,将肌腱分为拉伸区和压缩区,以定量羟脯氨酸和糖胺聚糖的含量。从完整肌腱的拉伸试验分析生物力学性能。在最大载荷和最大应力下进行生物力学测量。在笼养组和围栏组中,最大载荷和能量吸收随成熟而增加;然而,弹性模量、最大应力和最大应变并未随成熟而增加。运动导致第五个月的负载、应力和弹性模量增加。在围栏组中,胶原含量随年龄增加,而在第五和第八个月,运动导致胶原含量增加。运动导致年轻肌腱中糖胺聚糖的表达高于成熟肌腱。因此,低强度机械刺激促进了未成熟肌腱中分子的合成和可能的重排,而不活动则会在生长和成熟过程中对材料特性(最大应力和弹性模量)产生有害影响。