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一种新型头颈部鳞状细胞癌细胞系USC-HN1的建立与鉴定

Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1.

作者信息

Liebertz Daniel J, Lechner Melissa G, Masood Rizwan, Sinha Uttam K, Han Jing, Puri Raj K, Correa Adrian J, Epstein Alan L

机构信息

Department of Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, CA 90033, USA.

出版信息

Head Neck Oncol. 2010 Feb 22;2:5. doi: 10.1186/1758-3284-2-5.

DOI:10.1186/1758-3284-2-5
PMID:20175927
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2841166/
Abstract

BACKGROUND

Head and neck squamous cell carcinoma (HNSCC) is an aggressive and lethal malignancy. Publically available cell lines are mostly of lingual origin, or have not been carefully characterized. Detailed characterization of novel HNSCC cell lines is needed in order to provide researchers a concrete keystone on which to build their investigations.

METHODS

The USC-HN1 cell line was established from a primary maxillary HNSCC biopsy explant in tissue culture. The immortalized cells were then further characterized by heterotransplantation in Nude mice; immunohistochemical staining for relevant HNSCC biomarkers; flow cytometry for surface markers; cytogenetic karyotypic analysis; human papillomavirus and Epstein-Barr virus screening; qRT-PCR for oncogene and cytokine analysis; investigation of activated, cleaved Notch1 levels; and detailed 35,000 gene microarray analysis.

RESULTS

Characterization experiments confirmed the human HNSCC origin of USC-HN1, including a phenotype similar to the original tumor. Viral screening revealed no HPV or EBV infection, while western blotting displayed significant upregulation of activated, cleaved Notch1.

CONCLUSIONS

USC-HN1, a novel immortalized cell line has been derived from a maxillary HNSCC. Characterization studies have shown that the cell line is of HNSCC origin and displays many of the same markers previously reported in the literature. USC-HN1 is available for public research and will further the investigation of HNSCC and the development of new therapeutic modalities.

摘要

背景

头颈部鳞状细胞癌（HNSCC）是一种侵袭性致死性恶性肿瘤。公开可用的细胞系大多源自舌部，或未经过仔细鉴定。需要对新型HNSCC细胞系进行详细鉴定，以便为研究人员提供开展研究的具体关键基础。

方法

USC-HN1细胞系由上颌原发性HNSCC活检外植体在组织培养中建立。然后通过在裸鼠体内进行异种移植；对相关HNSCC生物标志物进行免疫组织化学染色；对表面标志物进行流式细胞术分析；细胞遗传学核型分析；人乳头瘤病毒和爱泼斯坦-巴尔病毒筛查；对癌基因和细胞因子进行定量逆转录聚合酶链反应分析；检测活化的、裂解的Notch1水平；以及进行详细的35000基因微阵列分析，对永生化细胞进行进一步鉴定。

结果

鉴定实验证实了USC-HN1源自人HNSCC，包括与原始肿瘤相似的表型。病毒筛查显示未感染人乳头瘤病毒或爱泼斯坦-巴尔病毒，而蛋白质免疫印迹显示活化的、裂解的Notch1显著上调。

结论

USC-HN1是一种源自上颌HNSCC的新型永生化细胞系。鉴定研究表明该细胞系源自HNSCC，并显示出许多先前文献报道的相同标志物。USC-HN1可用于公共研究，将推动HNSCC的研究及新治疗方法的开发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/76c38e4e0f53/1758-3284-2-5-9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/c0f12a99bd1e/1758-3284-2-5-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/8d93e1a32faf/1758-3284-2-5-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/a030b75d5c00/1758-3284-2-5-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/633ea959ef7a/1758-3284-2-5-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/8db983d26692/1758-3284-2-5-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/c6878bf750d4/1758-3284-2-5-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/8d8e3da8d973/1758-3284-2-5-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/e9f486922a69/1758-3284-2-5-8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/76c38e4e0f53/1758-3284-2-5-9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/c0f12a99bd1e/1758-3284-2-5-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/8d93e1a32faf/1758-3284-2-5-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/a030b75d5c00/1758-3284-2-5-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/633ea959ef7a/1758-3284-2-5-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/8db983d26692/1758-3284-2-5-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/c6878bf750d4/1758-3284-2-5-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/8d8e3da8d973/1758-3284-2-5-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/e9f486922a69/1758-3284-2-5-8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beae/2841166/76c38e4e0f53/1758-3284-2-5-9.jpg

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