Department of ENT, Da Nang Hospital, Da Nang, Vietnam.
Otolaryngol Head Neck Surg. 2010 Mar;142(3 Suppl 1):S27-32. doi: 10.1016/j.otohns.2009.09.022.
To determine whether or not endoscopic sinus surgery (ESS) alters bacterial biofilms in patients with chronic rhinosinusitis (CRS) after three months of follow-up.
Prospective study.
Department of Otorhinolaryngology-Head and Neck Surgery, University of Queensland.
Participants with CRS from the Princess Alexandra Hospital Department of Otorhinolaryngology-Head and Neck Surgery and from Logan Hospital were enrolled in the study (2008-2009). The total number of patients was 28, and the age range was 18 to 65 years. All patients underwent pre- and post-ESS questionnaires, endoscopic scoring, and nasal swabs for bacterial culture. Crystal violet staining was used to assess biofilm formation on a 96-well culture plaque.
ESS resulted in a statistically significant improvement in quality-of-life, subjective, and objective outcome measures after three months. A significant reduction was observed in biofilm density before and after ESS (2.63 versus 1.3, P = 0.043). No correlations between the reduction of bacterial biofilms with any of the subjective, objective, and quality-of-life outcomes were seen in our study.
ESS was shown to be capable of reducing the prevalence of bacterial biofilms but did not eliminate them entirely. There was no correlation between altered biofilm formation and improved outcome measures in individual patients.
确定内镜鼻窦手术 (ESS) 是否会改变慢性鼻鼻窦炎 (CRS) 患者在三个月随访后的细菌生物膜。
前瞻性研究。
昆士兰大学耳鼻喉科。
本研究纳入了来自Princess Alexandra 医院耳鼻喉科和Logan 医院的 CRS 患者（2008-2009 年）。总共有 28 名患者，年龄在 18 至 65 岁之间。所有患者均接受了 ESS 前后问卷调查、内镜评分和鼻拭子细菌培养。结晶紫染色用于评估 96 孔培养板上生物膜的形成。
ESS 在三个月后在生活质量、主观和客观结果测量方面产生了统计学上显著的改善。ESS 前后生物膜密度显著降低（2.63 与 1.3，P = 0.043）。在我们的研究中，没有观察到细菌生物膜减少与任何主观、客观和生活质量结果之间存在相关性。
ESS 能够降低细菌生物膜的流行率，但不能完全消除它们。在个别患者中，生物膜形成的改变与改善的结果测量之间没有相关性。
