桑野蚕中肠和唾液腺α-淀粉酶的酶学特性。（鳞翅目：螟蛾科）
Enzymatic properties of alpha-amylase in the midgut and the salivary glands of mulberry moth, Glyphodes pyloalis Walker (Lepidoptera: Pyralidae).
机构信息
Department of Plant Protection, University of Guilan, Rast Ghazvin Road, PO Box. 41635, 41635, Rasht, Iran.
出版信息
C R Biol. 2010 Jan;333(1):17-22. doi: 10.1016/j.crvi.2009.11.004. Epub 2010 Jan 25.
The pyralid moth, Glyphode pyloalis Walker, is an important pest of the mulberry. Amylases are the hydrolytic enzymes that catalyze the hydrolysis of the alpha-D-(1,4)-glucan linkage in glycogen and other related carbohydrates. Laboratory-reared fifth stadium larvae were randomly selected; the midgut (MG) and the salivary glands (SG) were removed by dissection under a dissecting microscope and alpha-amylase activity was assayed using the dinitrosalicylic acid procedure. The activity of alpha-amylase in the MG and the SG were 0.011 and 0.0018 micromol/min, respectively. The optimal pH and temperature for alpha-amylase were 9 for MG at 37-40 degrees C and 10 for SG at 37 degrees C respectively. Various concentrations of compounds (NaCl, KCl, MgCl(2), Urea, EDTA, SDS and CaCl(2)) had differential effects on the enzyme activity. Plant amylase inhibitors may play an important role against insect pests. Hence, the characterization of digestive enzymes and the examination of their inhibitors may be a useful tool in future management of this important mulberry pest.
果野蚕， Glyphode pyloalis Walker，是桑树的重要害虫。淀粉酶是一种水解酶，能催化糖原和其他相关碳水化合物中α-D-（1，4）-葡聚糖键的水解。随机选择实验室饲养的五龄幼虫；在解剖显微镜下通过解剖取出中肠（MG）和唾液腺（SG），并使用二硝基水杨酸法测定α-淀粉酶活性。MG 和 SG 中的α-淀粉酶活性分别为 0.011 和 0.0018 μmol/min。MG 的最适 pH 和温度分别为 9 和 37-40°C，SG 的最适 pH 和温度分别为 10 和 37°C。不同浓度的化合物（NaCl、KCl、MgCl2、尿素、EDTA、SDS 和 CaCl2）对酶活性有不同的影响。植物淀粉酶抑制剂可能在防治昆虫方面发挥重要作用。因此，消化酶的特性及其抑制剂的研究可能是未来防治这种重要桑树害虫的有用工具。
Zotero 插件