Richens Joanna L, Urbanowicz Richard A, Metcalf Rebecca, Corne Jonathan, O'Shea Paul, Fairclough Lucy
Cell Biophysics Group, School of Biology, University of Nottingham, UK.
J Biomol Screen. 2010 Jun;15(5):562-8. doi: 10.1177/1087057110362099. Epub 2010 Feb 22.
The focus of biomarker studies is shifting toward deciphering patterns of biomolecules as they provide a more comprehensive depiction of disease than individual biomarkers. Multiplexing technologies are crucial in deciphering such patterns, but it is essential that they are validated for reproducibility and precision to ensure accurate protein identification. Here the authors examine such properties in Cytokine Bead Array (CBA) and Luminex kits and compare concentration measurements to those obtained using enzyme-linked immunosorbent assay (ELISA). Luminex kits were found to be highly reproducible and reliable; however, CBA kits were not due to aberrant standards. Absolute cytokine concentrations were dependent on the detection kit, but correlations with ELISA were good for all technologies.
生物标志物研究的重点正转向解读生物分子模式，因为与单个生物标志物相比，它们能更全面地描述疾病。多重分析技术在解读此类模式方面至关重要，但必须对其进行可重复性和精确性验证，以确保准确的蛋白质识别。在此，作者研究了细胞因子微珠阵列（CBA）和Luminex试剂盒的此类特性，并将浓度测量结果与使用酶联免疫吸附测定（ELISA）获得的结果进行比较。发现Luminex试剂盒具有高度的可重复性和可靠性；然而，由于标准品异常，CBA试剂盒并非如此。细胞因子的绝对浓度取决于检测试剂盒，但所有技术与ELISA的相关性都很好。
