百日咳毒素敏感的 G 蛋白非依赖途径参与血清淀粉样蛋白 A 诱导的甲酰肽受体 2 介导的 CCL2 产生。
A pertussis toxin sensitive G-protein-independent pathway is involved in serum amyloid A-induced formyl peptide receptor 2-mediated CCL2 production.
机构信息
Department of Biological Science, Sungkyunkwan University, Suwon 440-746, Korea.
出版信息
Exp Mol Med. 2010 Apr 30;42(4):302-9. doi: 10.3858/emm.2010.42.4.029.
Abstract
Serum amyloid A (SAA) induced CCL2 production via a pertussis toxin (PTX)-insensitive pathway in human umbilical vein endothelial cells (HUVECs). SAA induced the activation of three MAPKs (ERK, p38 MAPK, and JNK), which were completely inhibited by knock-down of formyl peptide receptor 2 (FPR2). Inhibition of p38 MAPK and JNK by their specific inhibitors (SB203580 and SP600125), or inhibition by a dominant negative mutant of p38 MAPK dramatically decreased SAA-induced CCL2 production. Inactivation of G((i)) protein(s) by PTX inhibited the activation of SAA-induced ERK, but not p38 MAPK or JNK. The results indicate that SAA stimulates FPR2-mediated activation of p38 MAPK and JNK, which are independent of a PTX-sensitive G-protein and are essential for SAA-induced CCL2 production.
摘要
血清淀粉样蛋白 A（SAA）通过百日咳毒素（PTX）不敏感途径诱导人脐静脉内皮细胞（HUVEC）中 CCL2 的产生。SAA 诱导三种 MAPK（ERK、p38 MAPK 和 JNK）的激活，该激活可被甲酰肽受体 2（FPR2）敲低完全抑制。通过其特异性抑制剂（SB203580 和 SP600125）抑制 p38 MAPK 和 JNK，或通过 p38 MAPK 的显性负突变体抑制，均可显著降低 SAA 诱导的 CCL2 产生。PTX 使 G(i)蛋白失活可抑制 SAA 诱导的 ERK 的激活，但不抑制 p38 MAPK 或 JNK 的激活。结果表明，SAA 刺激 FPR2 介导的 p38 MAPK 和 JNK 的激活，该激活不依赖于 PTX 敏感的 G 蛋白，是 SAA 诱导 CCL2 产生所必需的。
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本文引用的文献
1
International Union of Basic and Clinical Pharmacology. LXXIII. Nomenclature for the formyl peptide receptor (FPR) family.国际基础和临床药理学联合会. LXXIII. 趋化因子受体（FPR）家族命名法。
Pharmacol Rev. 2009 Jun;61(2):119-61. doi: 10.1124/pr.109.001578. Epub 2009 Jun 4.
2
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Biochem Biophys Res Commun. 2009 Mar 6;380(2):313-7. doi: 10.1016/j.bbrc.2009.01.068. Epub 2009 Jan 22.
3
Serum amyloid A induces G-CSF expression and neutrophilia via Toll-like receptor 2.血清淀粉样蛋白A通过Toll样受体2诱导粒细胞集落刺激因子表达和中性粒细胞增多。
Blood. 2009 Jan 8;113(2):429-37. doi: 10.1182/blood-2008-03-139923. Epub 2008 Oct 24.
4
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5
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Exp Mol Med. 2008 Apr 30;40(2):237-45. doi: 10.3858/emm.2008.40.2.237.
6
Is serum amyloid A an endogenous TLR4 agonist?血清淀粉样蛋白A是一种内源性Toll样受体4激动剂吗？
J Leukoc Biol. 2008 May;83(5):1174-80. doi: 10.1189/jlb.0407203. Epub 2008 Feb 5.
7
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8
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Arterioscler Thromb Vasc Biol. 2007 Jul;27(7):1645-50. doi: 10.1161/ATVBAHA.106.137455. Epub 2007 Apr 26.
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