Patil Smita, Pore Y V, Kuchekar B S, Mane Aruna, Khire V G
Department of Pharmaceutical Chemistry, Government College of Pharmacy, Karad, 415 124, India.
Indian J Pharm Sci. 2009 Jan;71(1):58-61. doi: 10.4103/0250-474X.51961.
An accurate, specific and precise assay level gradient reverse-phase high-performance liquid chromatographic method was developed for simultaneous determination of montelukast sodium and bambuterol hydrochloride in tablet dosage form. An inertsil ODS C-18, 5 mum column having 250x4.6 mm I.D. in gradient mode, with mobile phase A, containing 0.025 M sodium phosphate buffer: methanol (85:15) and mobile phase B, containing acetonitrile:methanol (85:15) was used at different time intervals. The flow rate was 1.5 ml/min and effluent was monitored at 218 nm. The retention times of montelukast sodium and bambuterol hydrochloride were 21.2 min and 5.8 min respectively. The linearity for both the drugs was in the range of 0.25-0.75 mg/ml with correlation coefficients of 0.9999 and 0.9996 for montelukast sodium and bambuterol hydrochloride, respectively.
建立了一种准确、特异且精密的测定片剂剂型中孟鲁司特钠和盐酸班布特罗含量的梯度反相高效液相色谱法。采用内径为250×4.6 mm、粒径为5μm的Inertsil ODS C-18柱，在梯度模式下，不同时间间隔使用流动相A（含0.025 M磷酸钠缓冲液：甲醇，85:15）和流动相B（含乙腈：甲醇，85:15）。流速为1.5 ml/min，在218 nm处监测流出物。孟鲁司特钠和盐酸班布特罗的保留时间分别为21.2分钟和5.8分钟。两种药物的线性范围均为0.25 - 0.75 mg/ml，孟鲁司特钠和盐酸班布特罗的相关系数分别为0.9999和0.9996。
