Laboratoire de Bio Physiologie Cellulaires, Faculté des Sciences de Bizerte, Zarzouna, 7021 Bizerte, Tunisia.
Biol Trace Elem Res. 2010 Dec;138(1-3):326-36. doi: 10.1007/s12011-010-8631-3. Epub 2010 Feb 23.
The aim of this study is to investigate the effect of aluminium treatment on peroxidases activities and protein content in both soluble and cell-wall-bound fractions of sunflower leaves, stems and roots. Fourteen-day-old seedlings, grown in a nutrient solution, were exposed to a toxic amount of aluminium (500 μM AlNO(3)) for 72 h. Under stress conditions, biomass production, root length and leaf expansion were significantly reduced. Also, our results showed modulations on soluble and ionically cell-wall-bound peroxidases activities. In soluble fraction, peroxidases activities were enhanced in all investigated organs. This stimulation was also observed in ionically cell-wall-bound fraction in leaves and stems. Roots showed a differential behaviour: peroxidase activity was severely reduced. Lignifying peroxidases activities assayed using coniferyl alcohol and H(2)O(2) as substrates were also modulated. Significant stimulation was shown on soluble fraction in leaves, stems and roots. In ionically cell-wall-bound fraction lignifying peroxidases were enhanced only in stems but severely inhibited in roots. Also, aluminium toxicity caused significant increase on cell wall protein content in sunflower roots.
本研究旨在探讨铝处理对向日葵叶片、茎和根中过氧化物酶活性和蛋白质含量的影响。将在营养溶液中生长的 14 天大的幼苗暴露于有毒量的铝（500 μM AlNO（3））中 72 小时。在应激条件下，生物量生产、根长和叶片扩张显著减少。此外，我们的结果还显示了可溶性和离子结合细胞壁过氧化物酶活性的调节。在可溶性部分，所有研究的器官中的过氧化物酶活性都增强了。这种刺激在叶片和茎中的离子结合细胞壁部分也观察到了。根表现出不同的行为：过氧化物酶活性严重降低。用松柏醇和 H（2）O（2）作为底物测定木质素过氧化物酶活性也被调节。在叶片、茎和根的可溶性部分中显示出显著的刺激。在离子结合细胞壁部分中，木质素过氧化物酶仅在茎中增强，但在根中严重抑制。此外，铝毒性导致向日葵根细胞壁蛋白含量显著增加。
