Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bangalore 560064, India.
In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):200-5. doi: 10.1007/s11626-010-9277-3. Epub 2010 Feb 23.
We had earlier reported the derivation and characterization of two new sibling human embryonic stem cell lines BJNhem19 and BJNhem20, from discarded grade III embryos of Indian origin. We report here the characteristics of the two sibling cell lines after long-term continuous culture for over 2 yr during which they have been passaged over 200 times. We show that both cell lines adapt well to culture on various mouse and human feeders as well as in feeder-free conditions. The cells show normal diploid karyotype and continue to express all pluripotency markers. Both cell lines differentiate to derivatives of all three germ layers in vitro. However as reported earlier, BJNhem19 is unable to generate teratomas in nude or SCID mice or differentiate to beating cardiomyocytes when tested over several passages during long-term stable culture. On the other hand, the cardiac differentiation capacity of BJNhem20 is greatly increased, and it can generate beating cardiomyocytes that proliferate when isolated and cultured further. In conclusion, the two cell lines have maintained a stable phenotype for over 2 yr and are indeed immortal. Their derivation from grade III embryos does not seem to have any adverse effect on their long-term phenotype. The cells can be obtained for research purposes from the UK Stem Cell Bank and from the authors.
我们曾报道过源自印度废弃三级胚胎的两个新的人类胚胎干细胞系 BJNhem19 和 BJNhem20 的衍生和特性。我们在此报告两个同胞细胞系在经过超过 2 年的长期连续培养后的特性，在此期间它们已传代超过 200 次。我们表明，这两个细胞系在各种小鼠和人类饲养层上以及无饲养层条件下都能很好地适应培养。这些细胞表现出正常的二倍体核型，并继续表达所有多能性标记。两个细胞系都能在体外分化为三个胚层的衍生物。然而，正如之前报道的那样，BJNhem19 无法在裸鼠或 SCID 小鼠中生成畸胎瘤，也无法在长期稳定培养的多个传代中分化为搏动性心肌细胞。另一方面，BJNhem20 的心脏分化能力大大增强，它可以生成搏动性心肌细胞，这些细胞在进一步分离和培养时会增殖。总之，这两个细胞系在 2 年多的时间里保持了稳定的表型，确实是永生的。它们源自三级胚胎，这似乎对其长期表型没有任何不利影响。这些细胞可以从英国干细胞库和作者处获得用于研究目的。
