人急性白血病中 XAGE-1 基因的 mRNA 表达。

mRNA expression of the XAGE-1 gene in human acute leukemia.

机构信息

Department of Clinical Hematology, Second Hospital of Xi'an Jiaotong University, 157# West 5th Road, Xi'an, Shanxi, China.

出版信息

Int J Hematol. 2010 Mar;91(2):209-12. doi: 10.1007/s12185-010-0527-7. Epub 2010 Feb 24.

DOI:10.1007/s12185-010-0527-7

PMID:20178013

Abstract

XAGE-1 is a cancer-testis antigen, which was demonstrated to be expressed at a significant frequency and to be immunogenic in some solid tumors. We analyzed the expression of 4 XAGE-1 transcript variant gene in human acute leukemias by reverse-transcription polymerase chain reaction. Among the 114 acute leukemias, 14/63 (22.22%) of the acute myeloid leukemia samples were positive for XAGE-1b genes. XAGE-1b mRNA expression was detected in 10/51 (19.61%) of the acute lymphocyte leukemia samples. However, we did not find any important correlation between XAGE-1b mRNA expression and clinical characteristics, such as sex, leukemia type, response to therapy and the percentage of blast in the first diagnosed bone marrow. We concluded that the XAGE-1b gene was expressed at the mRNA level in a proportion of human acute leukemia.

摘要

XAGE-1 是一种癌睾丸抗原，在一些实体肿瘤中被证明具有较高的表达频率和免疫原性。我们通过逆转录聚合酶链反应分析了 4 种 XAGE-1 转录变体基因在人类急性白血病中的表达。在 114 例急性白血病中，63 例急性髓系白血病样本中有 14 例（22.22%）为 XAGE-1b 基因阳性。在 51 例急性淋巴细胞白血病样本中检测到 XAGE-1b mRNA 表达。然而，我们没有发现 XAGE-1b mRNA 表达与性别、白血病类型、治疗反应和首次诊断骨髓中原始细胞的百分比等临床特征之间存在任何重要相关性。我们的结论是，XAGE-1b 基因在一部分人类急性白血病中以 mRNA 水平表达。