具有不同长度src基因缺失的劳氏肉瘤病毒转化缺陷型突变体。
Transformation-defective mutants of Rous sarcoma virus with src gene deletions of varying length.
作者信息
Kawai S, Duesberg P H, Hanafusa H
出版信息
J Virol. 1977 Dec;24(3):910-4. doi: 10.1128/JVI.24.3.910-914.1977.
Abstract
The RNAs of transformation-defective (td) deletion mutants of the Schmidt-Ruppin strain of Rous sarcoma virus were found to vary in size when compared by polyacrylamide gel electrophoresis. Three of seven td mutants appeared to recombine with a mutant of Rous sarcoma virus (Schmidt-Ruppin), which has a temperature-sensitive sarcoma (src) gene and is termed ts68, to give rise to recombinants with a reduced temperature sensitivity. The results suggested that different clones of td mutants exist: some in which the src gene appears to be deleted, and others in which the src gene is only partially deleted. A direct correlation between RNA size and the extent of src gene deletion measured by recombination was not obtained, possibly because the recombination assay could only detect src sequences homologous to the lesion(s) of ts68, whereas the electrophoretic analysis of the RNA measured src deletions as well as other possible alterations of the RNA.
摘要
在通过聚丙烯酰胺凝胶电泳进行比较时，发现劳氏肉瘤病毒施密特-鲁平株的转化缺陷（td）缺失突变体的RNA大小各异。七个td突变体中的三个似乎与劳氏肉瘤病毒（施密特-鲁平株）的一个突变体重组，该突变体具有温度敏感型肉瘤（src）基因，被称为ts68，从而产生温度敏感性降低的重组体。结果表明存在不同的td突变体克隆：一些克隆中src基因似乎被删除，而另一些克隆中src基因只是部分被删除。未获得RNA大小与通过重组测量的src基因缺失程度之间的直接相关性，这可能是因为重组分析只能检测到与ts68损伤同源的src序列，而RNA的电泳分析测量的是src缺失以及RNA的其他可能改变。
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