具有不同长度src基因缺失的劳氏肉瘤病毒转化缺陷型突变体。
Transformation-defective mutants of Rous sarcoma virus with src gene deletions of varying length.
作者信息
Kawai S, Duesberg P H, Hanafusa H
出版信息
J Virol. 1977 Dec;24(3):910-4. doi: 10.1128/JVI.24.3.910-914.1977.
Abstract
The RNAs of transformation-defective (td) deletion mutants of the Schmidt-Ruppin strain of Rous sarcoma virus were found to vary in size when compared by polyacrylamide gel electrophoresis. Three of seven td mutants appeared to recombine with a mutant of Rous sarcoma virus (Schmidt-Ruppin), which has a temperature-sensitive sarcoma (src) gene and is termed ts68, to give rise to recombinants with a reduced temperature sensitivity. The results suggested that different clones of td mutants exist: some in which the src gene appears to be deleted, and others in which the src gene is only partially deleted. A direct correlation between RNA size and the extent of src gene deletion measured by recombination was not obtained, possibly because the recombination assay could only detect src sequences homologous to the lesion(s) of ts68, whereas the electrophoretic analysis of the RNA measured src deletions as well as other possible alterations of the RNA.
摘要
在通过聚丙烯酰胺凝胶电泳进行比较时，发现劳氏肉瘤病毒施密特-鲁平株的转化缺陷（td）缺失突变体的RNA大小各异。七个td突变体中的三个似乎与劳氏肉瘤病毒（施密特-鲁平株）的一个突变体重组，该突变体具有温度敏感型肉瘤（src）基因，被称为ts68，从而产生温度敏感性降低的重组体。结果表明存在不同的td突变体克隆：一些克隆中src基因似乎被删除，而另一些克隆中src基因只是部分被删除。未获得RNA大小与通过重组测量的src基因缺失程度之间的直接相关性，这可能是因为重组分析只能检测到与ts68损伤同源的src序列，而RNA的电泳分析测量的是src缺失以及RNA的其他可能改变。
相似文献
1
Transformation-defective mutants of Rous sarcoma virus with src gene deletions of varying length.具有不同长度src基因缺失的劳氏肉瘤病毒转化缺陷型突变体。
J Virol. 1977 Dec;24(3):910-4. doi: 10.1128/JVI.24.3.910-914.1977.
2
Mechanisms for the generation of src-deletion mutants and recovered sarcoma viruses: identification of viral sequences involved in src deletions and in recombination with c-src sequences.src缺失突变体和恢复的肉瘤病毒的产生机制：鉴定参与src缺失以及与c-src序列重组的病毒序列。
Virology. 1984 Oct 30;138(2):236-45. doi: 10.1016/0042-6822(84)90348-9.
3
Identification of the viral sequence required for the generation of recovered avian sarcoma viruses and characterization of a series of replication-defective recovered avian sarcoma viruses.鉴定产生恢复性禽肉瘤病毒所需的病毒序列，并对一系列复制缺陷型恢复性禽肉瘤病毒进行特性分析。
J Virol. 1984 Mar;49(3):881-91. doi: 10.1128/JVI.49.3.881-891.1984.
4
src Genes of ten Rous sarcoma virus strains, including two reportedly transduced from the cell, are completely allelic; putative markers of transduction are not detected.十种劳氏肉瘤病毒毒株的src基因（包括据报道从细胞中转导而来的两种）完全等位；未检测到转导的推定标记。
J Virol. 1981 Sep;39(3):758-76. doi: 10.1128/JVI.39.3.758-776.1981.
5
Recovery of avian sarcoma virus from tumors induced by transformation-defective mutants.从转化缺陷型突变体诱导的肿瘤中恢复禽肉瘤病毒。
J Exp Med. 1977 Dec 1;146(6):1735-47. doi: 10.1084/jem.146.6.1735.
6
Recombination between viral and cellular sequences generates transforming sarcoma virus.病毒序列与细胞序列之间的重组产生了转化性肉瘤病毒。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5812-6. doi: 10.1073/pnas.75.12.5812.
7
Induction of tumors and generation of recovered sarcoma viruses by, and mapping of deletions in, two molecularly cloned src deletion mutants.通过两个分子克隆的src缺失突变体诱导肿瘤并产生恢复的肉瘤病毒，以及对缺失进行定位
J Virol. 1984 Jun;50(3):904-13. doi: 10.1128/JVI.50.3.904-913.1984.
8
Analysis of the src gene of sarcoma viruses generated by recombination between transformation-defective mutants and quail cellular sequences.对由转化缺陷型突变体与鹌鹑细胞序列之间重组产生的肉瘤病毒src基因的分析。
J Virol. 1979 Nov;32(2):546-56. doi: 10.1128/JVI.32.2.546-556.1979.
9
Evidence for the common origin of viral and cellular sequences involved in sarcomagenic transformation.参与肉瘤发生转化的病毒和细胞序列共同起源的证据。
J Virol. 1980 Jul;35(1):52-64. doi: 10.1128/JVI.35.1.52-64.1980.
10
Occurrence of partial deletion and substitution of the src gene in the RNA genome of avian sarcoma virus.禽肉瘤病毒RNA基因组中src基因的部分缺失和取代现象。
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4781-5. doi: 10.1073/pnas.74.11.4781.
引用本文的文献
1
Development of FRET Biosensor to Characterize CSK Subcellular Regulation.CSK 亚细胞调控的荧光共振能量转移生物传感器的研制。
Biosensors (Basel). 2024 Apr 20;14(4):206. doi: 10.3390/bios14040206.
2
Dissection of the catalytic and regulatory structure-function relationships of Csk protein tyrosine kinase.Csk蛋白酪氨酸激酶的催化和调节结构-功能关系剖析
Front Cell Dev Biol. 2023 Mar 1;11:1148352. doi: 10.3389/fcell.2023.1148352. eCollection 2023.
3
NEDD9 sustains hexokinase expression to promote glycolysis.NEDD9维持己糖激酶表达以促进糖酵解。
Oncogenesis. 2022 Apr 11;11(1):15. doi: 10.1038/s41389-022-00391-w.
4
Identification of phosphotyrosine-containing proteins in untransformed and Rous sarcoma virus-transformed chicken embryo fibroblasts.未转化和劳斯肉瘤病毒转化的鸡胚成纤维细胞中含磷酸酪氨酸蛋白的鉴定。
Mol Cell Biol. 1982 Jun;2(6):653-65. doi: 10.1128/mcb.2.6.653-665.1982.
5
Characterization of a Rous sarcoma virus mutant defective in packaging its own genomic RNA: biological properties of mutant TK15 and mutant-induced transformants.一种在包装自身基因组RNA方面存在缺陷的劳氏肉瘤病毒突变体的特性：突变体TK15及突变体诱导的转化体的生物学特性
J Virol. 1984 Jul;51(1):147-53. doi: 10.1128/JVI.51.1.147-153.1984.
6
Induction of tumors and generation of recovered sarcoma viruses by, and mapping of deletions in, two molecularly cloned src deletion mutants.通过两个分子克隆的src缺失突变体诱导肿瘤并产生恢复的肉瘤病毒，以及对缺失进行定位
J Virol. 1984 Jun;50(3):904-13. doi: 10.1128/JVI.50.3.904-913.1984.
7
Identification of the viral sequence required for the generation of recovered avian sarcoma viruses and characterization of a series of replication-defective recovered avian sarcoma viruses.鉴定产生恢复性禽肉瘤病毒所需的病毒序列，并对一系列复制缺陷型恢复性禽肉瘤病毒进行特性分析。
J Virol. 1984 Mar;49(3):881-91. doi: 10.1128/JVI.49.3.881-891.1984.
8
Involvement of directly repeated sequences in the generation of deletions of the avian sarcoma virus src gene.直接重复序列参与禽肉瘤病毒src基因缺失的产生。
J Virol. 1983 Aug;47(2):380-2. doi: 10.1128/JVI.47.2.380-382.1983.
9
Small deletion in src of Rous sarcoma virus modifying transformation phenotypes: identification of 207-nucleotide deletion and its smaller product with protein kinase activity.劳氏肉瘤病毒src基因中的小缺失改变转化表型：207个核苷酸缺失及其具有蛋白激酶活性的较小产物的鉴定
J Virol. 1983 Jun;46(3):985-92. doi: 10.1128/JVI.46.3.985-992.1983.
10
Transformation-defective Rous sarcoma virus mutants with altered p19 of the gag gene and their inhibitory effect on host cell growth.具有gag基因p19改变的转化缺陷型劳氏肉瘤病毒突变体及其对宿主细胞生长的抑制作用。
J Virol. 1983 Jun;46(3):974-84. doi: 10.1128/JVI.46.3.974-984.1983.
本文引用的文献
1
Isolation of defective mutant of avian sarcoma virus.禽肉瘤病毒缺陷突变体的分离
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3493-7. doi: 10.1073/pnas.70.12.3493.
2
Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species.禽白血病和肉瘤病毒RNA在甲酰胺中的凝胶电泳：与其他病毒和细胞RNA种类的比较
J Virol. 1973 Sep;12(3):594-9. doi: 10.1128/JVI.12.3.594-599.1973.
3
RNA species obtained from clonal lines of avian sarcoma and from avian leukosis virus.从禽肉瘤克隆系和禽白血病病毒中获得的RNA种类。
Virology. 1973 Jul;54(1):207-19. doi: 10.1016/0042-6822(73)90130-x.
4
Genetic recombination with avian tumor virus.与禽肿瘤病毒的基因重组。
Virology. 1972 Jul;49(1):37-44. doi: 10.1016/s0042-6822(72)80005-9.
5
The a subunit in the RNA of transforming avian tumor viruses. I. Occurrence in different virus strains. II. Spontaneous loss resulting in nontransforming variants.转化禽肿瘤病毒RNA中的α亚基。I. 在不同病毒株中的存在情况。II. 自发缺失导致非转化变体。
Virology. 1972 Feb;47(2):494-7. doi: 10.1016/0042-6822(72)90287-5.
6
Spontaneous segregation of nontransforming viruses from cloned sarcoma viruses.非转化病毒从克隆肉瘤病毒中的自发分离。
Virology. 1971 Dec;46(3):939-46. doi: 10.1016/0042-6822(71)90092-4.
7
The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant.温度的相互变化对感染劳氏肉瘤病毒突变体的细胞转化状态的影响。
Virology. 1971 Nov;46(2):470-9. doi: 10.1016/0042-6822(71)90047-x.
8
Studies on the reproductive and cell-converting abilities of avian sarcoma viruses.禽肉瘤病毒的繁殖及细胞转化能力研究。
Virology. 1971 Feb;43(2):427-41. doi: 10.1016/0042-6822(71)90315-1.
9
Size and genetic content of viral RNAs in avian oncovirus-infected cells.禽肿瘤病毒感染细胞中病毒RNA的大小和遗传内容。
J Virol. 1977 Oct;24(1):47-63. doi: 10.1128/JVI.24.1.47-63.1977.
10
Distinguishable transformation-defective phenotypes among temperature-sensitive mutants of Rous sarcoma virus.劳氏肉瘤病毒温度敏感突变体中可区分的转化缺陷表型。
J Virol. 1977 Mar;21(3):1042-55. doi: 10.1128/JVI.21.3.1042-1055.1977.
Zotero 插件