Faculty of Medicine, Institute of Physiology, University of Coimbra, Coimbra, Portugal.
Br J Nutr. 2010 Jul;104(1):76-82. doi: 10.1017/S0007114510000164. Epub 2010 Feb 24.
Inflammation plays an important role in diabetes mellitus and its complications. In this context, the negative cross-talk between adipose tissue and skeletal muscle leads to disturbances in muscle cell insulin signalling and induces insulin resistance. Because several studies have shown that energy restriction brings some benefits to diabetes, the aim of the present study was to evaluate the effects of dietary restriction on systemic and skeletal muscle inflammatory biomarkers, such C-reactive protein, adipokines and cytokines, and in insulin resistance in Goto-Kakizaki rats. This is an animal model of spontaneous non-obese type 2 diabetes with strongly insulin resistance and without dyslipidaemia. Animals were maintained during 2 months of dietary restriction (50 %) and were killed at 6 months of age. Some biochemical determinations were done using ELISA and Western blot. Data from the present study demonstrate that in Goto-Kakizaki rats the dietary restriction improved insulin resistance, NEFA levels and adipokine profile and ameliorated inflammatory cytokines in skeletal muscle. These results indicate that dietary restriction in type 2 diabetes enhances adipose tissue metabolism leading to an improved skeletal muscle insulin sensitivity.
炎症在糖尿病及其并发症中起着重要作用。在这种情况下，脂肪组织和骨骼肌之间的负性交互作用导致肌肉细胞胰岛素信号转导紊乱，并诱导胰岛素抵抗。由于几项研究表明能量限制对糖尿病有一些益处，本研究旨在评估饮食限制对全身性和骨骼肌炎症生物标志物（如 C 反应蛋白、脂肪因子和细胞因子）以及 Goto-Kakizaki 大鼠胰岛素抵抗的影响。这是一种自发性非肥胖 2 型糖尿病动物模型，具有强烈的胰岛素抵抗而没有血脂异常。动物在饮食限制（50%）期间维持 2 个月，并在 6 个月时处死。使用 ELISA 和 Western blot 进行了一些生化测定。本研究的数据表明，在 Goto-Kakizaki 大鼠中，饮食限制改善了胰岛素抵抗、NEFA 水平和脂肪因子谱，并改善了骨骼肌中的炎症细胞因子。这些结果表明，2 型糖尿病中的饮食限制增强了脂肪组织代谢，从而提高了骨骼肌胰岛素敏感性。
