Dept. Measurement Technology and Industrial Electrical Engineering, Div. Nanobiotechnology, Lund University, 22100 Lund, Sweden.
Chem Soc Rev. 2010 Mar;39(3):1203-17. doi: 10.1039/b915999c. Epub 2010 Feb 4.
The progress in microfabrication and lab-on-a-chip technologies has been a major area of development for new approaches to bioanalytics and integrated concepts for cell biology. Fundamental advances in the development of elastomer based microfluidics have been driving factors for making microfluidic technology available to a larger scientific community in the past years. In line with this, microfluidic separation of cells and particles is currently developing rapidly where key areas of interest are found in designing lab-on-a-chip systems that offer controlled microenvironments for studies of fundamental cell biology. More recently industrial interests are seen in the development of micro chip based flow cytometry technology both for preclinical research and clinical diagnostics. This critical review outlines the most recent developments in microfluidic technology for cell and particle separation in continuous flow based systems. (130 references).
微制造和芯片实验室技术的进步一直是生物分析新方法和细胞生物学集成概念的主要发展领域。过去几年来，基于弹性体的微流控技术的发展取得了重大进展，这也是推动微流控技术能够为更广泛的科学界所接受的主要因素。与此相适应，微流控技术在细胞和颗粒的分离方面发展迅速，其中一个关键的研究领域是设计能够为基础细胞生物学研究提供可控微环境的芯片实验室系统。最近，人们对基于微芯片的流动细胞术技术的发展产生了浓厚的兴趣，无论是在临床前研究还是临床诊断方面。本文批判性地综述了基于连续流的微流控芯片技术在细胞和颗粒分离方面的最新进展。（参考文献 130 篇）
