Department of Genetics, Development and Cell Biology, Iowa State University, 2208, Molecular Biology Building, Ames, IA 50011-3260, USA.
Plant Cell Rep. 2010 Apr;29(4):413-7. doi: 10.1007/s00299-010-0832-x. Epub 2010 Feb 24.
We studied the products of alternative transposition reactions that utilize reverse-oriented Ds termini as substrates. In this configuration, Ds transposition can generate genome rearrangements including deletions, inversions, and reciprocal translocations. In approximately half of the transposition products recovered in Arabidopsis, the termini of the reversed ends Ds element were ligated together. The sequences at these fused-end junctions suggest that the excised transposon termini form covalently closed hairpin structures. These results shed new light on the mechanism of Ac/Ds transposition.
我们研究了利用反向定向 Ds 末端作为底物的替代转座反应产物。在这种构象下，Ds 转座可以产生包括缺失、倒位和相互易位在内的基因组重排。在拟南芥中回收的转座产物中，大约有一半的反向末端 Ds 元件的末端被连接在一起。这些融合末端连接处的序列表明，切除的转座子末端形成共价闭合的发夹结构。这些结果为 Ac/Ds 转座的机制提供了新的线索。
