Alalayah W M, Kalil M S, Kadhum A A H, Jahim J M, Jaapar S Z S, Alauj N M
Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Malaysia.
Pak J Biol Sci. 2009 Nov 15;12(22):1462-7. doi: 10.3923/pjbs.2009.1462.1467.
A two-stage fermentation process consisting of dark and photo-fermentation periods was carried out in a batch reactor. In the first stage, glucose was fermented in the dark stage using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564; CSN1-4) to produce acetate, CO2 and H2. The acetate produced in the first stage is fermented to H2 and CO2 by Rhodobacter sphaeroides NCIMB 8253 for further hydrogen production in the second, illuminated stage. The yield of hydrogen in the first stage was about 3.10 mol H2 (mol glucose)(-1) at a glucose concentration of 10 g L(-1), pH 6 +/- 0.2 and 37 degrees C and the second stage yield was about 1.10-1.25 mol H2 (mol acetic acid)(-1) at pH 6.8 +/- 0.2 and 32 degrees C, without removal of the Clostridium CSN1-4. The overall yield of hydrogen in the two-stage process, with glucose as the main substrate was higher than single-stage fermentation.
在间歇式反应器中进行了一个由黑暗发酵期和光发酵期组成的两阶段发酵过程。在第一阶段，利用丙酮丁醇梭菌N1-4（ATCC 13564；CSN1-4）在黑暗阶段对葡萄糖进行发酵，产生乙酸盐、二氧化碳和氢气。第一阶段产生的乙酸盐在第二阶段光照条件下被球形红杆菌NCIMB 8253发酵为氢气和二氧化碳，以进一步产氢。在葡萄糖浓度为10 g L(-1)、pH 6±0.2和37℃的条件下，第一阶段的氢气产率约为3.10 mol H2 (mol葡萄糖)(-1)；在pH 6.8±0.2和32℃的条件下，第二阶段的产率约为1.10-1.25 mol H2 (mol乙酸)(-1)，且未去除丙酮丁醇梭菌CSN1-4。以葡萄糖为主要底物的两阶段过程中氢气的总产率高于单阶段发酵。
